ARS | Publication request: A Genetic Map of Fusarium Graminearum Using Sequenced-Tagged Sites and Aflp Markers

Submitted to: American Phytopathological Society Abstracts
Publication Acceptance Date: July 31, 2004
Publication Date: August 4, 2004
Citation: Gale, L.R., Bryant, J.D., Giese, H., Katan, T., O Donnell, K., Suga, H., Usgaard, T.R., Ward, T.J., Kistler, H.C. A Genetic Map Of Fusarium Graminearum Using Sequenced-Tagged Sites And Aflp Markers. American Phytopathological Society Abstracts. 2004.

Technical Abstract: A genetic map of Fusarium graminearum was constructed using a cross between nitrate non-utilizing mutants of strain PH-1 (NRRL 31084) and a strain from Minnesota, 00-676 (NRRL 34097). A total of 111 ascospore progeny were analyzed for 237 genetic loci; 213 markers exhibited Mendelian inheritance, while segregation distortion was observed for 17 markers at four genomic locations. Genetic markers consisted of SNPs (detected as dCAPs, n=86 or CAPs, n=47), AFLPs (n=71), SSRs (n=27), and six other markers. A linkage map was generated using JoinMap 3.0. At a LOD score of 4.0, 11 linkage groups were obtained, anchoring many of the supercontigs that were generated for PH-1 by the whole-genome sequencing project (www.broad.mit.edu). All linkage groups and anchored supercontigs could be assembled into four chromosomes. A near-perfect match between the map location of the sequenced-tagged sites and their physical location in the genome were observed. The current map covers 1154 cM. Comparison of the physical and genetic maps revealed areas of chromosomes where recombination appears to be suppressed, which may point to centromere locations, while other regions appear to be hot-spots of recombination.