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Research Project: Crop Improvement Through Directed Genetic Recombination

Location: Plant Gene Expression Center (Albany, Ca)

Title: Transgene Management Via Multiple Site-Specific Recombination Systems

Author

Submitted to: In Vitro Plant
Publication Acceptance Date: July 1, 2004
Publication Date: August 1, 2004
Publisher's URL: www.sivb.org/i1543-706X-40-01a-26.pdf
Citation: Ow, D.W. 2004. Transgene Management Via Multiple Site-Specific Recombination Systems. In Vitro Plan, Abstract File 1543-706X-40-01A-26.PDF.

Interpretive Summary: Current methods for creating transgenic varieties are labor and time intensive, comprising of the generation of hundreds of plants with random DNA insertions, screening for the few individuals with appropriate transgene expression and simple integration structure, and followed by a lengthy breeding process to introgress the engineered trait into cultivated varieties. Various modifications of existing methods have been proposed to speed up the different steps involved in plant transformation, as well as a few add-on technologies that seek to address issues related to biosafety or intellectual property. This paper presents an integrated strategy for plant transformation, where the introduced DNA will be inserted precisely into the genome, the transgenic locus will be introgressed rapidly into field varieties, the extraneous transgenic DNA will be removed, the transgenic plants will be molecularly tagged, and the transgenic locus may be excised from pollen and/or seed.

Technical Abstract: Current methods for creating transgenic varieties are labor and time intensive, comprising of the generation of hundreds of plants with random DNA insertions, screening for the few individuals with appropriate transgene expression and simple integration structure, and followed by a lengthy breeding process to introgress the engineered trait into cultivated varieties. Various modifications of existing methods have been proposed to speed up the different steps involved in plant transformation, as well as a few add-on technologies that seek to address issues related to biosafety or intellectual property. The problem with an assortment of independently developed improvements is that they do not integrate seamlessly into a single transformation system. This paper presents an integrated strategy for plant transformation, where the introduced DNA will be inserted precisely into the genome, the transgenic locus will be introgressed rapidly into field varieties, the extraneous transgenic DNA will be removed, the transgenic plants will be molecularly tagged, and the transgenic locus may be excised from pollen and/or seed.

 
Project Team
Ow, David

Publications

Related National Programs
  Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)
  Plant Biological and Molecular Processes (302)

Related Projects
   Engineering Seed Specific Expression of a Gossypol Degradation Gene

 
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