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Research Project:
Molecular & Genetic Analyses of Pollen Developmentand Pollen-Pistil Signaling in Crop Plants
Location:
Plant Gene Expression Center (Albany, Ca)
Title: A Compendium of Methods Useful for Characterizing Arabidopsis Pollen Mutants and Gametophytically-Expressed Genes
Authors
Submitted to: Plant Journal
Publication Acceptance Date: June 15, 2004
Publication Date: August 30, 2004
Publisher's URL: www.blackwell-synergy.com/servlet/useragent?func=showIssues&code;=tpj
Citation: Johnson-Brousseau, S., Mccormick, S.. 2004. A Compendium Of Methods Useful For Characterizing Arabidopsis Pollen Mutants And Gametophytically-Expressed Genes. Plant Journal, 39(5):761-775.
Interpretive Summary: This article provides detailed protocols for collecting pollen and outlines genetic crosses and phenotypic assays that are useful for characterizing mutants that affect pollen development.
Technical Abstract: Here we provide detailed protocols for preparing flowers for microscopic observation of pollen and for collecting pollen in bulk for germination or for RNA or protein isolation. We outline crossing schemes that will help confirm or refute hypothesized roles for the gene of interest, and we outline phenotypic characterizations that are useful in determining whether a gene product is important during one or more phases of pollen development or germination.
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