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2nd Edition

Primary Containment for Biohazards:

Selection, Installation and
Use of Biological Safety Cabinets


U.S. Department of Health and Human Services
Public Health Service
Centers for Disease Control and Prevention
and
National Institutes of Health

September 2000

2nd Edition now available in .pdf format.

Note: Accompanying tables and figures are not included in PDF but are included separately below.

Editors Contributors

SECTION 1 - Introduction

SECTION 2 - The High Efficiency Particulate Air (HEPA) Filter and the Development of Biological Containment Devices

SECTION 3 - Biological Safety Cabinet

SECTION 4 - Laboratory Hazards and Risk Assessment

SECTION 5 - BSC Use by the Investigator: Practices and Procedures

SECTION 6 - Facility and Engineering Requirements

SECTION 7 - Certification of Biological Safety Cabinets

ACKNOWLEDGMENTS
REFERENCES

TABLES

Table 1. Selection of a Safety Cabinet Through Risk Assessment
Table 2. Comparison of Biosafety Cabinet Characteristics
Table 3. Performance Tests to be AppLIed to the Three Classes
Table 4. References for AppLIcable Containment Tests

FIGURES

Figure 1. HEPA filters
Figure 2. The Class I BSC
Figure 3. The Class II, Type A BSC
Figure 4. Thimble Unit
Figure 5A. The Class II, Type B1 BSC (classic design)
Figure 5B. The Class II, Type B1 BSC (bench top design)
Figure 6. The Class II, Type B2 BSC
Figure 7. The tabletop model of a Class II, Type B3 BSC
Figure 8. The Class III BSC
Figure 9A. The horizontal laminar flow "clean bench"
Figure 9B. The vertical laminar flow "clean bench"
Figure 10. A modified containment cabinet or Class I BSC
Figure 11. A typical layout for working "clean to dirty"
Figure 12. One method to protect a house vacuum system
Figure 13. A bag-in-out filter enclosure

Office of Health and Safety, Centers for Disease Control and Prevention,
1600 Clifton Road N.E., Mail Stop F05 Atlanta, Georgia 30333, USA
Last Modified: 5/17/2001
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