Human BAC Resource

Integration of cytogenetic landmarks into the draft sequence of the human genome

The BAC Resource consortium

Nature 409, 953-958 (2001)

This resource provides genome-wide resource of large-insert clones that will help integrate cytogenetic, radiation-hybrid, linkage, and sequence maps of the human genome.

Table 1. A cytogenetic resource of FISH-mapped, sequence-tagged clones (HBRC_1 set). Click number in the table to view list of clones

⁺Clones are placed on NCBI contigs. Only clones that are localiized to one or two places on the same chromosome on the draft sequence are included in this count This table was last updated on Fri Aug 27 16:39:57 EDT 2004.

This set, HBRC_1 set, initially consisted of 8877 clones but the number has been changed as more FISH data became available. This set consists of primarily bacterial artificial chromosome (BAC) clones.

- Each clone has been mapped by Fluorescent In Situ Hybridization (FISH).

- Each clone has one or more sequence tags which can be used to anchor the clone onto the human genomic sequence map. Sequence tags are in the form of either GenBank accession number from actual draft or finished sequence of clone insert DNA, GenBank accession numbers from BAC-end sequences, or STS determined by PCR and hybridization experiment.

- Each clone is available as single-colony purified bacterial stock through one of three distributors.

Figure 1b.

Clones selected on the basis of band location were used in FISH analysis to map the breakpoint of a translocation involving chromosomes 11 and 19 in a patient with multiple congenital malformations and mental retardation (DGAP012, http://dgap.harvard.edu). Clone CTD-3193o13 spans the breakpoint on chromosome 19; red signal is split between the derivative chromosome 11 and derivative 19 chromosomes and is also present on the normal chromosome 19. The GTG-banded karyotype for this patient is 46,X,Y,t(11;19)(p11.2;p13.3).

The BAC Resource Consortium

V.G.Cheung^1*, N.Nowak^2*, W.Jang³, I.R.Kirsch⁴, S.Zhao⁵, X.-N.Chen⁶, T.S.Furey¹⁰, U.-J.Kim^7%, W.-L.Kuo⁸, M.Olivier⁹, J.Conroy², A.Kasprzyk¹¹, H.Massa¹², R.Yonescu⁴, S.Salt², C.Thoreen^13%, A.Snijders⁸, E.Lemyre¹⁴, J.A.Bailey¹⁵, A.Bruzel¹, W.D.Burill¹¹, S.M.Clegg¹¹, S.Collins¹³, P.Dhami¹¹, C.Friedman¹², C.S.Han¹⁶, S.Herrick¹⁴, J.Lee⁷, A.H.Ligon¹⁴, S.Lowrey¹⁷, M.Mortey¹, S.Naraslmban¹, K.Osoegawa¹⁸, Z.Peng¹⁷, I.Plajzer-Frick¹⁷, B.J.Quade¹⁴, D.Scott¹⁷, K.Sirotkin³, A.A.Thorpe¹¹, J.W.Gray⁸, J.Hudson¹⁹, D.Pinkel⁸, T.Ried⁴, L.Rowen²⁰, G.L.Shen-Ong^4%, R.I.Strausberg⁴, E.Birney¹¹, D.F.Callen²¹, J.-F.Cheng¹⁷, D.R.Cox⁹, N.A.Doggett¹⁶, N.P.Carter¹¹, E.E.Eichler¹⁵, D.Haussler¹⁰, J.R.Korenberg⁶, C.C.Morton¹⁴, D.Albertson⁸, G.Schuler³, P. de Jong¹⁸, and B.J.Trask¹²

^* equal contributions

Addresses:
1, Department of Pediatrics, University of Pennsylvania, The Children's Hospital of Philadelphia, 3516 Civic Center Blvd., ARC 516, Philadelphia PA 19104;

2, Roswell Park Cancer Institute, Elm and Carlton Street, Buffalo NY 14263;

3, National Center for Biotechnology Information, National Library of Medicine, Building 38A/Room 8N805, Bethesda MD 20894;

4, National Cancer Institute, NIH, Building 10/Room 12N214, Bethesda MD 20889-5105;

5, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville MD 20850;

6, Departments of Pediatrics and Human Genetics, Cedars-Sinai Medical Center, 8700 Beverly Boulevard, Los Angeles CA 90048;

7, Department of Biology, California Institute of Technology, Mail Code 147-75, Pasadena CA 91125;

8, University of California San Francisco Cancer Center, Box 0808, San Francisco CA 94143-0808;

9, Stanford University, Genome Lab, Mail Code 5120, Stanford CA 94305-5120;

10, Computer Science Department, University of California Santa Cruz, 1156 High Street, Santa Cruz CA 95064-1077;

11, Sanger Center, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK;

12, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North C3-168, P.O. Box 19024, Seattle WA 98109-1024;

13, Department of Molecular Biotechnology, University of Washington, Box 357730, Seattle WA 98195-7730;

14, Departments of Obstetrics and Gynecology and Pathology, Brigham and Women's Hospital, Amory Lab Building 3rd floor, Boston MA 02115;

15, Department of Human Genetics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland OH 44106;

16, Joint Genome Institute-Los Alamos National Laboratory, MS M888 B-N1, P.O. Box 1663, Los Alamos NM 87545;

17, Joint Genome Institute-Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mail Stop 84-171, Berkeley CA 94720;

18, Children's Hospital Oakland Research Institute, 747 52nd Street, Oakland CA 94609;

19, Research Genetics, 2130 Memorial Parkway, Huntsville AL 35801;

20, Institute for Systems Biology, 4225 Roosevelt Way NE, Suite 200, Seattle WA 98105-6099;

21, Department of Cytogenetics and Molecular Genetics, Women's and Children's Hospital, 72 King William Road, North Adelaide, South Australia 5006, Australia;

%Present addresses:

PanGenomics, 6401 Foothill Boulevard, Tujunga CA 91024 (U.-J. Kim);

Harvard Medical School, 240 Longwood Avenue, Cell Biology, Cambridge MA 02115 (C. Thoreen);

Gene Logic, Inc., 708 Quince Orchard Road, Gaithersburg MD 20878 (G. L. Shen-Ong).


Correspondence should be addressed to B.J.T. (e-mail: btrask@fhcrc.org).