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Research Project: New Technologies for Decontamination of Fresh Fruits and Vegetables Containing Human Pathogens

Location: Food Safety Intervention Technologies Research

Project Number: 1935-41420-007-00
Project Type: Appropriated

Start Date: Feb 14, 2001
End Date: Feb 13, 2006

Objective:
Develop more effective technologies for decontaminating fresh fruits and vegetables containing human pathogens by assessing factors limiting efficacy of conventional washing methods and equipment and developing new approaches to decontamination based on novel anti-microbial agents and physical treatments. Design, procure and evaluate novel decontamination equipment required for treatment application in a BL-2 pilot plant. Transfer effective decontamination technology to produce industry.

Approach:
The relative importance of microbial adhesion, inaccessibility, biofilm formation and internalization in limiting efficacy of sanitizing produce will be investigated. Novel anti-microbial treatments that attempt to overcome these factors will be evaluated with artificially inoculated produce using non-pathogenic surrogates of human pathogens. Efficacious treatments will be modified to permit evaluation with commercial-like washing equipment installed in a BL-2 pilot plant. Specialized equipment for treatment application will be designed, fabricated and evaluated with artificially inoculated produce. A containment and equipment decontamination system will be developed so that treatment efficacy can be confirmed with produce inoculated with human pathogens. New technology and recommendations for produce decontamination will be transferred to the produce industry, equipment manufacturers and government agencies by means of workshops, demonstrations, cooperative agreements, and publications.

 
Project Team
Sapers, Gerald
Ukuku, Dike
Fett, William
Annous, Bassam
Sites, Joseph

Project Annual Reports
  FY 2003
  FY 2002
  FY 2001

Publications

Related National Programs
  Food Safety, (animal and plant products) (108)

Related Projects
   Enhancing the Microbial Safety of Fresh and Fresh-Cut Melon
   Novel Bl-2 Processing System for Improving Safety of Fresh Fruit and Vegetable Products
   Efficacy of Chlorine Dioxide Gas in Inactivating Inaccessible Bacteria on Apples and Melons

 
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