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Research Project:
GERMPLASM ENHANCEMENT, GENETICS AND DISEASE MANAGEMENT OF COOL SEASON FOOD LEGUMES
Location:
Grain Legume Genetics Physiology Research
Project Number: 5348-21000-014-00
Project Type:
Appropriated
Start Date: Jul 01, 2003
End Date: Jun 30, 2008
Objective:
Develop enhanced pea, lentil and chickpea germplasm with resistance to biotic and abiotic stresses and with improved yield potential, plant architecture, and high end-use quality. Adapt winter hardy legumes to direct seeding cropping systems; Characterize the genomes of pea, lentil and chickpea including gene identification and mapping, with special emphasis on functional genomics. Identify and characterize genes for disease resistance, plant architecture and winter survival; Increase understanding of host-pathogen interactions and mechanisms of disease resistance to formulate strategies for managing grain legume diseases of economical importance.
Approach:
Cyclical hybridization including single, double and triple crosses will be used to combine favorable alleles for traits of interest. Parental lines will include adapted germplasm, commercial cultivars and germplasm accessions possessing specific traits of interest such as disease resistance, increased biomass production and seed yield. Progeny from specific hybridizations will be evaluated in F4 to F8 for acceptable agronomic characters and seed quality; We will rely on the use of genetically stable recombinant inbred lines (RILs) in pea, lentil and chickpea for mapping of genes of interest. These RIL populations will be developed through the single seed descent procedure starting with the F2 and proceeding to the F7 where we would expect to have nearly homozygous lines. Population sizes will be sufficient to allow quantitative trait loci analysis as well as the analysis of simply inherited traits. RIL populations of 200 to 300 will be developed for traits such as ascochyta blight resistance and winter hardiness in pea; Isolates of grain legume pathogens will be obtained from a wide range of geographic areas and from a variety of host genotypes including susceptible and known resistant cultivars. A permanent collection of 300 isolates of each pathogen under study will be established along with information about their geographic origins and host genotypes from which they were isolated. Pathogenicity of these isolates will be tested using appropriate bioassay techniques on resistant and susceptible cultivars.
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