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Display category headings
Research Project:
Identification, Tagging, and Characterization of Resistance Genes to Ppv in Prunus Species
Location:
Foreign Disease-Weed Science
Project Number: 1920-22000-028-01
Project Type:
Specific C/A
Start Date: Sep 01, 2001
End Date: Aug 31, 2005
Objective:
Utilize molecular marker systems to tag gene(s) controlling resistance to plum pox virus in apricot, thereby facilitating the introgression of these genes into other cultivars and potentially into other Prunus species; having identified markers close to the resistance genes, utilize this information in conjunction with structural and functional genomic efforts in peach and apricot to initiate cloning and characterization of the resistance genes.
Approach:
Utilizing the mapping populations identified by European colleagues, use amplified fragment length polymorphism (AFLP) technology on mapping populations of 'Stark Early Orange' and 'Harlayne' to identify markers linked to virus resistance. These markers will be mapped on different apricot molecular maps to determine the number of marked resistance loci. Using BAC libraries of apricot and peach, these markers will be converted to microsatellite markers to facilitate marker-assisted selection in breeding programs. Initiate cloning and identification of resistance genes and mobilize these genes into other cultivars and species through marker assisted breeding and transgenic technologies.
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