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James A Higgins
Environmental Microbial Safety

Res Microbiologist
jhiggins@anri.barc.usda.gov
Phone: (301) 504-6443
Fax: (301) 504-6608
Room 202
10300 BALTIMORE BLVD
BLDG 173 BARC-EAST
BELTSVILLE, MD, 20705-0000

Project List

Publications
item Threat Agents and Water Biosecurity- (30-Sep-04)
item Detection and Molecular Characterization of Enteric Pathogens in Fresh Water- (24-Oct-03)
item Real-Time Pcr Detection of B. Anthracis: Lessons from the Real World- (09-Oct-03)
item Surveying the Baltimore Ecosystem Study Stream Sites for the Presence of Enteric Pathogens- (05-Sep-03)
item Detection of Cryptosporidium Parvum in Polluted Stream Water- (01-Jun-03)
item Recovery and Detection of Cryptosporidium Parvum Oocysts from Water Samples Using Continuous Flow Centrifugation, Pcr, and Fluorescence in Situ Hybridization- (01-May-03)
item Molecular Tools to Evaluate the Epidemiology and Control of Waterborne Zoonotic Infections- (01-May-03)
item Prevalence and Molecular Characterization of Enteropathogenic E. Coli in Streams Along An Urban-Rural Gradient in the Baltimore, Maryland Metropolitan Area- (25-Feb-03)
item A Handheld Real Time Thermal Cycler for Bacterial Pathogen Detection- (19-Sep-02)
item A Field Investigation of Bacillus Anthracis Contamination of U.S. Department of Agriculture and Other Washington, D.C., Buildings During the Anthrax Attack of October 2001- (02-Apr-02)
item Real Time Pct for the Detection of Cryptosporidium Parvum- (01-Oct-01)
item Rapid Extracton of DNA from Escherichia Coli and Cryptosporidium Parvum for Use in Pcr- (23-Aug-01)
item Rapid Nucleic Acid Extraction from Eastern Oysters, Crassostera Virginica- (18-Sep-00)
item Exporting Novel Dod-Sponsored Technologies for Waterborne Pathogen Detection- (30-Apr-00)
item Molecular Epidemiology of Cyclospora and Cryptosporidium Infections among Us Military Personnel in Indonesia- (01-Nov-99)
item Detection of Francisella Tularensis in Infected Mammals and Vectors Using Probe-Based Pcr- (29-Oct-99)
item Evaluation of Pcr for Detection of Cryptosporidium Parvum in Environmental and Agricultural Samples- (11-Jul-99)
item Murine Flea-Borne Typhus- (07-Apr-99)
item Protection of Calves Against Cryptosporiosis by Oral Inoculation with Gamma-Irradiated Cryptosporidium Parvum Oocysts.- (14-May-04)
item Estimation of Viable Escherichia Coli 0157 in Surface Waters Using Enrichment in Conjunction with Immunological Detection- (26-Mar-04)
item Quantification of a Glyptapanteles Indiensis Polydnavirus Gene Expression in Its Parasitized Host, Lymantria Dispar by Real-Time Quantitative Rt-Pcr- (20-Aug-03)
item Mobile Laboratory for Bacillus Anthracis Detection- (16-May-02)
item Tracking Fecal Pathogens from Cattle and Wildlife in the Chesapeake Bay- (28-Mar-02)
item Bovine Enteroviruses Are Abundant in the Environment and Can Serve As a Marker for Fecal Contamination- (14-Dec-01)
item Pcr As a Diagnostic and Quantitative Technique in Veterinary Parasitology- (21-Jul-01)
item Evaluation of a Fluorogenic Real-Time Pcr Assay for the Detetion of Toxoplasma Gondii in Biological Samples- (23-Jul-00)
item Detection of Fate of Bacillus Anthracis (Sterne) Vegetative Cells and Spores Added to Bulk Tank Milk- (10-Jul-03)
item Advancing Cryptosporidium Parvum Detection Methodologies- (08-Aug-02)
item Transcriptional Analysis of Rickettsia Prowazekii Invasion Gene Homolog (Inva) During Host Cell Infection- (08-Aug-02)
item Comparison of Assays for Viable Cryptosporidium Parvum Oocysts Stored in Water at Constant Temperature- (11-Jul-02)
item A Real-Time Rt-Pcr Reaction Specific for Bovine Enteroviruses Provides a Rapid Assay for Fecal Contamination of the Environment by Cattle- (10-Jun-02)
item Prevalence of Giardia in Dairy Calves and Cross-Species Transmission Potential- (14-May-02)
item Mrna Levels for Amyloglucosidase As Measured by Rt-Pcr Correlate with Infectivity and Fecundity of Cryptosporidium Parvum Oocysts for Neonatal Balb/c Mice- (07-Jul-00)
item Molecular Cloning and Expression of DNA Sequence Encoding a Cryptosporidium Parvum Oocyst Wall Protein- (21-Dec-99)
item Fluorescent Real-Time Molecular (Pcr) Detection of Zoonotic Bacteria and Viruses in Bulk MILK.- (10-Jun-02)
item Molecular Epidemiology of Animal Enteroviruses: Implications for Their Use As Markers for Environmental Fecal Contamination- (28-Mar-02)
item Cryptosporidium, Giardia, and Microsporidia in Beef Cattle in Maryland- (30-Jun-01)
item Estimating Viability of Cryptosporidium Parvum Oocysts Using Reverse Transcriptase-Polymerase Chain Reaction Directed at Mrna Encoding Amyloglucosidase- (18-Aug-00)
item Susceptibility of the Chesapeake Bay to Environmental Contamination with Cryptosporidium Parvum- (01-Feb-00)
item Cloning and Expression of a DNA Sequence Encoding a 41 Kda Cryptosporidium Parvum Oocyst Wall Protein- (27-Sep-99)
item Human and Animal Pathogens in the Chesapeake Bay Watershed: Cryptosporidiumand Pfiesteria- (26-Sep-99)
item Cryptosporidium Parvum Identification in Oysters from Commercial Harvestingsites in the Chesapeake Bay- (02-Aug-99)
item Morphological and Molecular Characterization of Meloidogyne Floridae N. Sp. (Nematoda: Meloidogynidae), a Root-Knot Nematode Parasitizing Peach in Florida- (22-Apr-03)
item Western Regional Research Project W-102, Integrated Methods of Parasite- (27-Jul-00)
 
 
 
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