Project Number: 6202-22000-024-03
Project Type: Reimbursable

Start Date: Jan 01, 2004
End Date: Dec 31, 2004

Objective:
We will identify and clone the gene responsible for the conversion of desoxyhemigossypol to 4-hydroxydesoxyhemigossypol and then identify the upstream elements that regulate its expression.

Approach:
We will identify the gene responsible for the conversion of hemigossypol to 4-hydroxyhemigossypol using PCR and differential hybridization. We will prepare a degenerate primer corresponding to a conserved sequence upstream of the heme region from the P450 monooxygenase previously identified from cotton. After PCR amplification the cDNA will be inserted into E. coli. After amplification in E. coli, plasmid DNA will be spotted onto nictrocellulose membranes for hybridization. Probes will be generated from total RNAs isolated from leaves of near isogenic glanded and glandless G. hirsutum cultivars. Clones showing preferential hybridization to probes present in gladed cultivars that are lacking in glanded cultivars will be selected and sequenced.

Project Team

Stipanovic, Robert

Related National Programs

Plant Diseases (303)