Project Number: 6202-22000-024-04
Project Type: Reimbursable

Start Date: Jan 01, 2004
End Date: Dec 31, 2004

Objective:
Develop our existing germplasm with reniform nematode immunity inherited from Gossypium longicalyx sufficiently to make a definitive determination regarding its potential use in Upland cotton breeding, and simultaneously take important initial steps toward the development of molecular markers for the specific G. longicalyx gene(s) that confer the reniform-immunity trait.

Approach:
We will backcross to the BC6 generation, selecting for immunity (nematode reproduction <1% of the control) at each generation, and put the trait into at least three backgrounds. At each generation, 20 plants in each background will be screened and the three most agronomic immune plants in each will be retained. Thus, we expect to progress two generations a year, by evaluating approximately 200 plants plus controls at each generation, and achieve the BC6 generation of the slowest line by mid 2006. Since we already have BC3 seed from one cross, the most optimistic outcome possible would be to have the first BC6 seed by early 2005. Simultaneously, we will analyze selected progeny cytogenetically to guide marker development and breeding.

Project Team

Stipanovic, Robert

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Plant Diseases (303)