NSF LogoNSF Award Abstract - #0221880 AWSFL008-DS3

SGER: Interactions between Microbial Nitrogen Cycling and Acid Mine Drainage
Contaminants in Impacted Streams

NSF Org BES
Latest Amendment Date August 12, 2002
Award Number 0221880
Award Instrument Standard Grant
Program Manager Patrick L. Brezonik
BES DIV OF BIOENGINEERING & ENVIRON SYSTEMS
ENG DIRECTORATE FOR ENGINEERING
Start Date September 1, 2002
Expires August 31, 2004 (Estimated)
Expected Total Amount $77593 (Estimated)
Investigator JoAnn Silverstein joann.silverstein@colorado.edu (Principal Investigator current)
Sponsor U of Colorado Boulder
3100 Marine Street, Room 481
Boulder, CO 803090572 303/492-6221
NSF Program 1440 ENVIRONMENTAL ENGINEERING
Field Application 0316000 Trace Contaminants
0313040 Water Pollution
Program Reference Code 1440,1689,9187,9237,EGCH,

Abstract

0221880 Silverstein The effects of acid mine drainage on natural biogeochemical nitrogen cycling will be studied with respect to the interaction of microbial nitrogen and iron transformations at the lowered pH found in receiving waters. Competition between ferric iron and nitrate as electron acceptors, use of ferrous iron as electron donor in denitrification, and mobilization of iron and other heavy metals from bacterial reduction will be investigated in acid main drainage impacted streams. Species identification in impacted and unimpacted streams will be made utilizing DNA probes and the FISH technique and microscopic counts will be used to produce quantitative estimates of chemolithotrophic iron oxidizers and heterotrophic acidophiles.

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