NSF Award Abstract - #0221836| NSF Award Abstract - #0221836 |
AWSFL008-DS3 |
| NSF Org | BES |
| Latest Amendment Date | September 6, 2002 |
| Award Number | 0221836 |
| Award Instrument | Standard Grant |
| Program Manager |
Patrick L. Brezonik BES DIV OF BIOENGINEERING & ENVIRON SYSTEMS ENG DIRECTORATE FOR ENGINEERING |
| Start Date | September 1, 2002 |
| Expires | August 31, 2006 (Estimated) |
| Expected Total Amount | $563999 (Estimated) |
| Investigator | Michael D. Aitken mike_aitken@unc.edu (Principal Investigator current) |
| Sponsor |
U of NC Chapel Hill 104 Airport Drive Chapel Hill, NC 275991350 919/966-3411 |
| NSF Program | 1440 ENVIRONMENTAL ENGINEERING |
| Field Application |
0316000 Trace Contaminants 0313040 Water Pollution |
| Program Reference Code | 1440,1689,9187,EGCH, |
0221836 Aitken A stable isotope method will be used to prepare heavy DNA from incubations of mixed cultures and specific C-13 labelled PAH substrates in completely closed slurry aerobic bioreactors. The bioreactor is fitted with a gas chromatograph which allows on- line respirometric measurements. DNA and rRNA will be extracted from the slurry, amplified by PCR, and analyzed on agarose gels. Comparisons between the DGGE profiles obtainedfrom rDNA (PCR), rRNA (RT-PCR) and C-13 labelled rDNA and rRNA will provide insight into the metabolically active members of the community. Selected DGGE bands will be sequenced and submitted for phylogenetic analysis using PHYLIP software.
