Technical Abstract: Bovine enteroviruses are members of the Picornaviridae family, genus Enterovirus. Little is known about their pathogenic potential but they may be widely distributed in at least some cattle and cattle environments. Only one of the two current serotypes has been completely sequenced. In this report we present the entire genome sequences of the prototype European BEV-2 strain, (PS-87), and a recent isolate from an endemically infected herd in Maryland, (Wye-3A). The recent isolate clearly segregated phylogenetically with sequences representing the BEV-2 serotype, as did other isolates from the endemic herd. The Wye-3A isolate shared 82% nucleotide sequence identity with the prototype PS-87 strain and 68% identity with the prototype BEV-1 strain (VG-5-27). Comparison of BEV2 and BEV-1 deduced protein sequences revealed 72-73% identity and showed most differences to be single amino acid changes or single deletions with the exception of the VP-1 protein where both BEV-2 sequences were 7 amino acids shorter than that of BEV-1. Homology modeling of the capsid proteins of BEV-2 against protein database entries for picornaviruses indicated six significant differences between bovine enteroviruses and other picornaviridae. Five of these were on the 'rim' of the proposed enterovirus receptor binding site or 'canyon' (VP-1) and one near the base (VP-3) of the canyon. Two of these regions varied enough to distinguish the BEV-2 from the BEV-1 strains. This is the first report and analysis of full-length sequences for BEV-2. Continued analysis of these wild-type strains should yield useful information for genotyping enteroviruses and modeling enterovirus capsid structure.