Project Number: 3625-32000-062-06
Project Type: Specific C/A

Start Date: Sep 16, 2004
End Date: Jul 31, 2007

Objective:
The objective of this cooperative research project is to elucidate host immune response mechanisms for cattle during infection with Mycobacterium avium subsp. paratuberculosis.

Approach:
Peripheral blood mononuclear cells will be isolated from noninfected and naturally infected cattle and analyzed by flow cytometry for activation markers that may be involved in host immune responses. Because monoclonal antibodies for some cell activation markers may not be available for bovine, this project will encompass sequence identification of conserved regions of the gene, peptide synthesis, and monoclonal antibody production to the peptide of interest. Concurrent analyses will be performed to evaluate intracellular expression of cytokines that may be involved in host immune responses to M. paratuberculosis.

Project Team

Stabel, Judith Donald Beitz - Distinguished Professor Of Animal Science 516-663-7304

Related National Programs

Animal Health (103)