MedlinePlus Medical Encyclopedia: Dexamethasone suppression test

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Dexamethasone suppression test

Contents of this page:
Alternative names Definition How the test is performed How to prepare for the test How the test will feel	Why the test is performed Normal Values What abnormal results mean What the risks are Special considerations

Alternative names

DST; ACTH suppression test; Cortisol suppression test

Definition Return to top

Dexamethasone suppression test measures the response of the adrenal glands to ACTH. Dexamethasone is given and levels of cortisol are measured. Cortisol levels should decrease in response to the administration of dexamethasone.

How the test is performed Return to top

There are two different types of dexamethasone suppression tests: the low-dose test and the high-dose test. Each type can either be done in an overnight or standard (3-day) way.

For the low-dose overnight method, 1 mg of dexamethasone is given at 11 p.m., and the blood is drawn at 8 a.m. for a cortisol measurement (see venipuncture).
In the standard low-dose method, urine is collected at 24-hour intervals over 3 days for measurement of cortisol. On day 2, a low dose (0.5 mg) of dexamethasone is given by mouth every 6 hours for 48 hours.

For the high-dose overnight method, a baseline cortisol is measured on the morning of the test, then 8 mg of dexamethasone is given at 11 p.m.. Blood is drawn at 8 a.m. for a cortisol measurement. For the standard high-dose test, urine is collected at 24-hour intervals over 3 days for measurement of cortisol. On day 2, a high dose (2 mg) of dexamethasone is given by mouth every 6 hours for 48 hours.

How to prepare for the test Return to top

The health care provider may advise you to discontinue drugs that may affect the test. Drugs that can affect test results include barbiturates, estrogens, corticosteroids, oral contraceptives, phenytoin, spironolactone, and tetracyclines.

Infants and children:
The preparation you can provide for this test depends on your child's age, previous experiences, and level of trust. For general information regarding how you can prepare your child, see the following topics:

Infant test or procedure preparation (birth to 1 year)
Toddler test or procedure preparation (1 to 3 years)
Preschooler test or procedure preparation (3 to 6 years)
Schoolage test or procedure preparation (6 to 12 years)
Adolescent test or procedure preparation (12 to 18 years)

How the test will feel Return to top

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

Why the test is performed Return to top

This test is performed when overproduction of cortisol is suspected. The low-dose test can help differentiate healthy people from those who produce too much cortisol. The high-dose test can help determine if the abnormality is in the pituitary gland (Cushing's Disease).

The secretion of ACTH from the pituitary gland is normally regulated by the level of cortisol in the blood plasma. ACTH stimulates the adrenal cortex to produce cortisol. As plasma cortisol levels increase, ACTH secretion is suppressed. As cortisol levels decrease, ACTH increases.

Dexamethasone is a synthetic steroid similar to cortisol, which suppresses ACTH secretion in normal people. Therefore, giving dexamethasone should reduce ACTH levels, resulting in decreased cortisol levels. People with pituitary glands which produce too much ACTH will have an abnormal response to the low-dose test, but a normal response to the high dose.

Normal Values Return to top

Low Dose:

Overnight: 8 a.m. plasma cortisol< 5 mcg/dl
Standard: Urinary free cortisol on day 3 < 10 mcg/day

High Dose:

Overnight: > 50 % reduction in plasma cortisol
Standard: > 90% reduction in urinary free cortisol

Normal value ranges may vary slightly among different laboratories.

What abnormal results mean Return to top

If there is not a normal response on the low-dose test, abnormal secretion of cortisol is likely (Cushing's Syndrome). This could be a result of a cortisol-producing adrenal tumor, a pituitary tumor that produces ACTH, or a tumor in the body that inappropriately produces ACTH. The high-dose test can help distinguish a pituitary cause (Cushing's Disease) from the others.

Cushing's syndrome caused by adrenal tumor

Low dose: no change
High dose: no change

Cushing's syndrome related to ectopic ACTH-producing tumor

Low dose: no change
High dose: no change

Cushing's Syndrome caused by pituitary tumor (Cushing's Disease)

Low dose: no change
High dose: normal suppression

What the risks are Return to top

Excessive bleeding
Fainting or feeling lightheaded
Hematoma (blood accumulating under the skin)
Infection (a slight risk any time the skin is broken)
Multiple punctures to locate veins

Special considerations Return to top

There are none.

Update Date: 2/2/2004

Updated by: Tarun Jain, M.D., Endocrinology & Infertility Division, Brigham and Women's Hospital, Boston, MA. Review provided by VeriMed Healthcare Network.

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