NSF LogoNSF Award Abstract - #0321683 AWSFL008-DS3

Finishing the Rice Genome

NSF Org DBI
Latest Amendment Date June 28, 2004
Award Number 0321683
Award Instrument Continuing grant
Program Manager Jane Silverthorne
DBI DIV OF BIOLOGICAL INFRASTRUCTURE
BIO DIRECT FOR BIOLOGICAL SCIENCES
Start Date September 1, 2003
Expires July 31, 2006 (Estimated)
Expected Total Amount $4202799 (Estimated)
Investigator W. Richard McCombie mccombie@cshl.org (Principal Investigator current)
Rod A. Wing (Co-Principal Investigator current)
Yeisoo Yu (Co-Principal Investigator current)
Carol Soderlund (Co-Principal Investigator current)
Sponsor Cold Spring Harbor Lab
P.O. Box 100
Cold Spring Harbor, NY 11724 / -
NSF Program 1329 PLANT GENOME RESEARCH PROJECT
Field Application
Program Reference Code 9109,BIOT,

Abstract

Rice is the most important food source worldwide. The rice genome also serves as a model for the closely related crops such as corn and wheat. The corn and wheat genomes are much larger than that of rice so the rice genome provides a cost effective way to understand these other plants. The International Rice Genome Sequencing Project (IRGSP) completed a high quality draft sequence of the rice genome (Oryza sativa ssp. japonica, cv. Nipponbare) in December 2002 and is now committed to finishing the sequence

This project continues previous, successful work towards finishing the public rice genome sequence. The 300-350 Bacterial Artificial Chromosome (BAC) clones to be sequenced to finished standards (an error rate of no more than 1/10,000 bases) comprise about 10% of the genome. Support from this award will also allow the project to collaborate with groups from other countries to sequence 1% - 2% of the most technically challenging regions of the genome that remain to be completed. The project will host and train scientists from other countries participating in the IRGSP. Training opportunities will also be available for local high school students and faculty from minority serving institutions in a collaboration between the Cold Spring Harbor Genome Center and the Dolan DNA Learning Center.

Deliverables All sequence information will be released according to the Bermuda Agreement/Fort Lauderdale principles (http://www.genome.gov/page.cfm?pageID=10506537). All contiguous stretches of sequence (contigs) over 1,500 base pairs in length will be immediately and automatically released at http://nucleus.cshl.edu/riceweb/, including sequence and chromotogram files. Assemblies of BAC sequences will be updated weekly and released to GenBank. The BAC clones sequenced are available through the Clemson University BAC/EST Resource Center (http://www.genome.clemson.edu).


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