NSF LogoNSF Award Abstract - #0321701 AWSFL008-DS3

Apple Functional Genomics

NSF Org DBI
Latest Amendment Date June 28, 2004
Award Number 0321701
Award Instrument Continuing grant
Program Manager Jane Silverthorne
DBI DIV OF BIOLOGICAL INFRASTRUCTURE
BIO DIRECT FOR BIOLOGICAL SCIENCES
Start Date September 1, 2003
Expires August 31, 2005 (Estimated)
Expected Total Amount $1659708 (Estimated)
Investigator Schuyler S. Korban korban@uiuc.edu (Principal Investigator current)
Lei Liu (Co-Principal Investigator current)
Lila O. Vodkin (Co-Principal Investigator current)
Natalie Carroll (Co-Principal Investigator current)
Herbert S. Aldwinckle (Co-Principal Investigator current)
Sponsor U of Ill Urbana-Champaign
801 South Wright Street
Champaign, IL 61820 217/333-2186
NSF Program 1329 PLANT GENOME RESEARCH PROJECT
Field Application
Program Reference Code 9109,BIOT,

Abstract

Abstract The overall goal of this NSF project is to establish structural tools for apple genomics. The apple, Malus x domestica Borkh., is the most important deciduous tree fruit crop grown in the United States. This project will focus on developing structural genomic research tools with emphasis on flowering, fruiting, and pathogen response in apple. The first phase of this project is to construct an expressed sequence tag (EST) database of 120,000 sequences from flowering and fruiting tissues, and from tissues responding to pathogen infection, specifically to the bacterial pathogen Erwinia amylovora (the causal organism of fire blight disease) and to the fungal pathogen Venturia inaequalis (the causal organism of apple scab disease). These will then be used to generate a set of 30,000 unigene clones that will be subjected to 3' sequencing. An apple genome dataset will be generated and integrated into a national Rosaceae Genome Database. An educational and outreach component of this project will involve developing web-based modules for use in educating high school teachers and students on the concepts structural genomics.

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