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Biotechnology

Advances in DNA Analyses Help Identify 9/11 Victims

NIST staff members John Butler and Susan Ballou discuss the World Trade Center DNA project at a robotic sample preparation station. Copyright Robert Rathe

NIST staff members John Butler and Susan Ballou discuss the World Trade Center DNA project at a robotic sample preparation station.

Remains from 16 additional victims of the Sept. 11 attacks on the World Trade Center have been positively identified thanks in part to a new method for analyzing DNA developed with assistance from the National Institute of Standards and Technology (NIST). Based on a concept developed at NIST, the new method allows accurate identification of DNA samples that are too damaged or degraded to be analyzed with conventional procedures. The NIST research was funded by the National Institute of Justice.

The matching of more than 20,000 recovered bone and tissue remains from the World Trade Center site with DNA samples provided by relatives of presumed victims is the largest DNA identification effort ever undertaken. Remains from about half of the approximately 2,800 victims were identified previously with standard DNA analyses.

One of several advanced analysis techniques being used in the WTC identification effort, the new method reduces the size of DNA fragments needed for a positive identification. Conventional forensic methods using “short tandem repeats, or STRs” analyze DNA fragments from 13 specific locations within the human genome. Each fragment contains approximately 200 to 400 pairs of the nitrogen-containing compounds or “base pairs” that comprise the genetic code of an individual. The NIST approach uses fragments that come from the same 13 locations but are substantially smaller, with roughly 25 to 190 fewer base pairs depending on the location. The smaller size means that damaged or degraded DNA samples are more likely to have intact sections at each of the 13 specific locations. This research will be described in an upcoming issue of the Journal of Forensic Sciences.

The ongoing effort to identify additional damaged or degraded WTC remains is being carried out by a group of government agencies and industry contractors under the leadership of the New York City Medical Examiner’s Office.

Media Contact:
Laura Ost, (301) 975-4034

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Space

NIST Assists NASA in Columbia Accident Investigation

The National Institute of Standards and Technology (NIST) has provided significant assistance to the National Aeronautics and Space Administration (NASA) in its investigation of the space shuttle Columbia disaster on Feb. 1, 2003. NASA has drawn upon NIST’s expertise in cold temperature research for data on the properties of liquid and solid nitrogen and for measurements of thermal conductivity in foam insulation.

According to NASA, one theory for the disaster is that pieces of foam insulation broke off from the shuttle’s external tank shortly after liftoff and struck the leading edge of the Columbia’s left wing. This may have caused a failure in the insulation on the wing, which proved disastrous upon re-entry. According to the theory, liquid and solid nitrogen between the foam and the tank wall may have contributed to the breaking away of the foam from the fuel tank.

NIST was able to supply immediately the properties of liquid nitrogen from its cryogenic database, and it provided NASA with properties data on solid nitrogen from 20 Kelvin up to its melting temperature of 63 Kelvin after a search of relevant cryogenics research papers. NIST also helped NASA repair and calibrate an instrument for measuring the thermal conductivity of the foam at extremely cold temperatures, a critical part of the accident investigation tests. The unique instrument—a very low temperature guarded hot plate—was developed at NIST and later transferred to NASA’s Marshall Space Flight Center in Huntsville, Ala.

Media Contact:
Fred McGehan (Boulder), (303) 497-3246

 

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First Responders

A New Wireless Network for Emergency Communications

First responders would like to be able to send messages simultaneously to all the emergency workers at the scene of a disaster if necessary, but lack of interoperability among various types of radio equipment prevents them from doing so today. In the future, first responders converging on a disaster scene may be able to quickly and easily exchange emergency messages and data using a wireless ad hoc network recently developed and tested by scientists and engineers at the National Institute of Standards and Technology (NIST). NIST’s work in this area is part of the federal government’s efforts to improve first responder communications in light of the September 11 terrorist attacks.

The network consists of personal digital assistants (PDAs) equipped with wireless local area network (WLAN) cards. Transmission routes among the PDAs are established automatically and without need for networking infrastructure at the emergency site as the first responders arrive on the scene. The network may use any nearby PDA to relay messages to others at the scene and allows transmission of voice, text, video and sensor data. If a worker leaves the disaster scene or a device is destroyed, the network automatically reorganizes itself.

Small video screens can display the names of workers and their roles. In buildings equipped with radios at reference locations, the network would determine the locations of first responders and track their movements. The devices also could receive information from smoke, heat or vibration sensors embedded in smart buildings that could be transmitted by wireless sensor networks or distributed by first responders during emergencies.

Media Contact:
Philip Bulman, (301) 975-5661

 

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Security

Novel Spectroscopic Method Can Detect Terrorist Threats

A novel technique that uses far-infrared (terahertz ) radiation to rapidly identify bulk or airborne materials inside sealed paper or plastic containers has been demonstrated by scientists at the National Institute of Standards and Technology (NIST) and SPARTA Inc., of Rosslyn, Va. Described at a recent technical conference,* the technology has potential applications in homeland security such as detection of explosives in the mail or other non-metallic portable containers.

The method involves directing a far-infrared light source at a sample in a closed container, detecting the light transmitted through the materials, and then analyzing the light that was absorbed by the sample while making adjustments for the light absorbed by the container. Far-infrared radiation, which falls between visible light and radio waves on the electromagnetic spectrum, is partially transmitted through many materials. The pattern of light frequencies or spectra absorbed by a material depends specifically on the vibrations of the material’s atoms and its crystalline structure.

This method can readily identify compounds made of molecules containing three to hundreds of atoms, the size of many threat materials. The two instruments employed, one using a pulsed laser and the other a glowing filament, are tabletop-sized and work at room temperature. Two years of experiments have demonstrated that the technique detects aerosols (such as those that might contain anthrax spores), pharmaceutical powders, most gases, several explosives and other common materials. The researchers have compiled a database of spectral characteristics for more than 100 materials and developed an automated software tool for rapidly identifying bulk materials based on their absorption spectra. Further research aims to increase the sensitivity and throughput speed of the technology.

*Campbell, M.B. and Heilweil, E.J., “Non-invasive detection of weapons of mass destruction using THz radiation,” in Proceedings of SPIE Vol. 5070 Terahertz for Military and Security Applications, edited by R. Jennifer Hwu, Dwight Woolard, (SPIE, Bellingham, WA, 2003) in press.

Media Contact:
Laura Ost, (301) 975-4034

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Security

Ensuring the Reliability of Trace Explosive Detectors

In an effort to enhance homeland security, National Institute of Standards and Technology (NIST) chemists are developing new ways to “see” collections of micrometer-sized particles of explosive materials. The techniques will help ensure that equipment for screening airport passengers, baggage and cargo operates reliably.

The research is part of a collaboration with the Transportation Security Agency to develop metrology for designing, characterizing and calibrating trace explosive detection systems. Thousands of enhanced trace explosive detection systems already are deployed in airports to collect particles from luggage and other surfaces, and analyze these samples in a tabletop instrument. Research also is under way on new systems that will use “air showers” to blow particles off people as they walk through portals.

The detection of trace explosives involves three basic processes: particle collection on cloth or mesh filters, particle heating to produce vapors and vapor analysis to identify the chemicals. NIST is using its measurement expertise to develop methods to better understand and optimize particle collection and heating processes.

One set of NIST experiments focuses on how evenly current tabletop analytical instruments heat the filters that collect particles. Heat control is important because explosive particles need to get hot enough to vaporize within seconds for rapid analysis, and uneven heating may lead to variation in analytical results. To assist in the testing and design of walk-through portal systems, NIST scientists developed a way to visualize a few individual explosive particles surrounded by millions of background particles collected on filters. Additional NIST experiments focus on the size of particles knocked off a test surface by various air jet designs. Such information can be used to help design next-generation detection systems.

Editor’s note: Graphic available.

Media Contact:
Laura Ost, (301) 975-4034

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Quick Links

  • The Fifteenth Symposium on Thermophysical Properties, co-sponsored by the National Institute of Standards and Technology and the American Society of Mechanical Engineers, will be held June 22 to 27, 2003, at the University of Colorado, Boulder, Colo. Topics to be featured include the theoretical, experimental, simulation and applied aspects of the thermophysical properties of gases, liquids and solids, including biological systems. To register, visit the symposium Web site at http://symp15.nist.gov.

Editor:Gail Porter

Date created: 5/27/2003
Contact: inquiries@nist.gov