NSF LogoNSF Award Abstract - #0211661 AWSFL008-DS3

Collaborative Research: The Green Plant BAC Library Project

NSF Org IBN
Latest Amendment Date July 21, 2004
Award Number 0211661
Award Instrument Cooperative Agreement
Program Manager Judith Plesset
IBN DIV OF INTEGRATIVE BIOLOGY AND NEUROSCIE
BIO DIRECT FOR BIOLOGICAL SCIENCES
Start Date August 1, 2002
Expires July 31, 2005 (Estimated)
Expected Total Amount $1281539 (Estimated)
Investigator Rod A. Wing rwing@ag.arizona.edu (Principal Investigator current)
Carol Soderlund (Co-Principal Investigator current)
Jeffrey P. Tomkins (Co-Principal Investigator current)
Sponsor U of Arizona
601 Administration Building
Tucson, AZ 85721 602/621-2211
NSF Program 1111 DEVELOPMENTAL MECHANISMS PROG
Field Application 0000099 Other Applications NEC
Program Reference Code 1080,1619,9183,BIOT,

Abstract

0211661 Wing

This research project is designed to accomplish two aims: Aim 1: To construct and array high-quality BAC libraries to provide a genomic resource on a wide range of species. Aim 2: To enable researchers working with green algae, non-seed land plants, and seed plants (including flowering plants) to identify genes critical for understanding plant form and function and how land plants arose and diversified. This project will enable progress toward an understanding of the genetic basis for the transitions that mark the most fundamentally important steps in green plant evolution. The Deep Green community (http://ucjeps.herb.berkeley.edu/bryolab/deepgene/index.html) will help to provide an infrastructure for ongoing scientific exchange. Bioinformatics and a web site will be provided to the community to access these resources (http://www.genome.clemson.edu/).

Selected Species: Desired coverage is given in parentheses. Where two coverage values are listed, two libraries using different restriction enzymes will be made.

Green algae: Volvox carteri (5x, 5x) Caulerpa mexicana (8x) Mesostigma viride (8x) Coleochaete orbicularus (6.4x, 6.4x) Chara aspera (6x)

Non-seed plants: Marchantia polymorpha (8x) Anthoceros sp. (6.3x, 6.3x) Lycopodium lucidulum (5.1x, 5.1x) Angiopteris erecta (6.2x, 6.2x) Ceratopteris richardii (4.8x, 4.8x) Marsilea quadrifolia (5.6x, 5.6x) Seed plants: Amborella trichopoda (5.5x) Nuphar adventa (7x) Acorus gramineus (7x) Lirodendron tulipifera (7x) Mimulus guttatus (7x)


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