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National Guidance

Guidance for Assessing Chemical Contaminant Data for Use In Fish Advisories

Volume 1: Fish Sampling and Analysis - Third Edition

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    • Acronyms

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      1. Introduction (PDF, 311k)
      1.1 Historical Perspective
      1.2 Purpose
      1.3 Objectives
      1.4 Relationship of Manual to Other Guidance Documents
      1.5 Organization of this Manual

      2. Monitoring Strategy (PDF, 412k)
      2.1 Screening Studies (Tier 1)
      2.2 Intensive Studies (Tier 2)

      3. Target Species (PDF, 352k)
      3.1 Purpose of Using Target Species
      3.2 Criteria for Selecting Target Species
      3.3 Freshwater Target Species

      3.3.1 Target Finfish Species
      3.3.2 Target Turtle Species
      3.4 Estuarine/Marine Target Species
      3.4.1Target Shellfish Species
      3.4.2 Target Finfish Species

      4.Target Analytes (PDF, 603k)
      4.1 Recommended Target Analytes
      4.2 Selection of Target Analytes
      4.3 Target Analyte Profiles

      4.3.1Metals
      4.3.2 Organochlorine Pesticides
      4.3.3 Organophosphate Pesticides
      4.3.4 Chlorophenoxy Herbicides
      4.3.5 Polycyclic Aromatic Hydrocarbons (PAHs)
      4.3.6 Polychlorinated Biphenyls (Total)
      4.3.7 Dioxins and Dibenzofurans
      4.4 Target Analytes under Evaluation
      4.4.1 Lead

      5. Screening Values for Target Analytes (PDF, 94k)
      5.1 General Equations for Calculating Screening Values

      5.1.1 Noncarcinogens
      5.1.2 Carcinogens
      5.1.3 Recommended Values for Variables in Screening Value Equations
      5.2 Recommended Screening Values for Target Analytes
      5.3 Comparison of Target Analyte Concentrations with Screening Values
      5.3.1 Metals
      5.3.2 Organics

      6. Field Procedures (PDF, 964k)
      6.1 Sampling Design

      6.1.1 Screening Studies (Tier 1)
      6.1.2 Intensive Studies (Tier 2)
      6.2 Sample Collection
      6.2.1 Sampling Equipment and Use
      6.2.2 Preservation of Sample Integrity
      6.2.3 Field Recordkeeping
      6.3 Sample Handling
      6.3.1 Sample Selection
      6.3.2 Sample Packaging
      6.3.3 Sample Preservation
      6.3.4 Sample Shipping

      7. Laboratory Procedures I Sample Handling (PDF, 251k)
      7.1 Sample Receipt and Chain-of-Custody
      7.2 Sample Processing

      7.2.1 General Considerations
      7.2.2 Processing Fish Samples
      7.2.3 Processing Turtle Samples
      7.2.4 Processing Shellfish Samples
      7.3 Sample Distribution
      7.3.1 Preparing Sample Aliquots
      7.3.2 Sample Transfer

      8..Laboratory Procedures II Sample Analyses (PDF, 169k)
      8.1 Recommended Analytes

      8.1.1 Target Analytes
      8.1.2 Lipid
      8.2 Analytical Methods
      8.2.1 Lipid Method
      8.2.2 Target Analyte Methods
      8.3 Quality Assurance and Quality Control Considerations
      8.3.1 QA Plans
      8.3.2 Method Documentation
      8.3.3 Minimum QA and QC Requirements for Sample Analyses
      8.4 Documentation and Reporting of Data
      8.4.1 Analytical Data Reports
      8.4.2 Summary Reports

      9. Data Analysis and Reporting (PDF, 33k)
      9.1 Data Analysis

      9.1.1 Screening Studies
      9.1.2 Intensive Studies
      9.2 Data Reporting
      9.2.1 State Data Reports
      9.2.2 Reports to the National Fish Tissue Data Repository

      10. Literature Cited (PDF, 131k)

      Appendix

      A 1993 Fish Contaminant Workgroup (PDF, 7k)
      B Screening Values for Defining Green Areas (PDF, 26k)
      C Use of Individual Samples in Fish Contaminant Monitoring Programs (PDF, 42k)
      D Fish and Shellfish Species for which State Consumption Advisories Have Been Issued (PDF, 21k)
      E Target Analytes Analyzed in National or Regional Monitoring Programs (PDF, 83k)
      F Pesticides and Herbicides Recommended as Target Analytes (PDF, 28k)
      G Target Analyte Dose-Response Variables and Associated Information (PDF, 26k)
      H A Recommended Method for Inorganic Arsenic Analysis (PDF, 561k)
      I Quality Assurance and Quality Control Guidance (PDF, 13k)
      J Recommended Procedures for Preparing Whole Fish Composite Homogenate Samples (PDF, 54k)
      K General Procedures for Removing Edible Tissues from Freshwater Turtles (PDF, 80k)
      L General Procedures for Removing Edible Tissues from Shellfish (PDF, 368k)
      M M. Sources of Reference Materials and Standards (PDF, 23k)
      N Statistical Methods for Comparing Samples: Spatial and Temporal Considerations (PDF, 58k)

      LIST OF FIGURES

      1-1 Total number of fish advisories in effect in each state in 1998 (change from 1997)
      1-2 Trends in number of advisories issued for various pollutants
      1-3 Series summary: Guidance for assessing chemical contamination data for use in fish advisories
      2-1 Recommended strategy for State fish and shellfish contaminant monitoring programs
      3-1 Geographic range of the common snapping turtle (Chelydra serpentina
      3-2 Geographic distributions of three bivalve species used extensively in national contaminant monitoring programs
      4-1 States issuing fish and shellfish advisories for mercury
      4-2 States issuing fish and shellfish advisories for chlordane
      4-3 States issuing fish and shellfish advisories for PCBs
      4-4 States issuing fish and shellfish advisories for dioxin/furans
      5-1 Recommended Values for Mean Body Weights (Bws) and Fish Consumption Rates (CRs) for Selected Subpopulations
      5-2 Fish Consumption Rates for Various Fisher Populations
      5-3 Dose-Response Variables and Recommended Screening Values (SVs) for Target Analytes - Recreational Fishers
      5-4 Dose-Response Variables and Recommended Screening Values (SVs) for Target Analytes - Subsistence Fishers
      5-5 Example Screening Values (SVs) for Various Target Populations and Risk Levels (RLs)
      5-6 Toxicity Equivalency Factors for Various PAHs
      5-7 Toxicity Equivalency Factors (TEFs) for Tetra-through Octa-Chlorinated Dibenzo-p-Dioxins and Dibenzofurans and Dioxin-Like PCBs
      6-1 Example of a sample request form
      6-2 U.S. fish and wildlife service regions
      6-3 Example of a field record for fish contaminant monitoring program--screening study
      6-4 Example of a field record for shellfish contaminant monitoring progra m-- screening study
      6-5 Example of a field record for fish contaminant monitoring program-- intensive study
      6-6 Example of a field record for shellfish contaminant monitoring program -- intensive study
      6-7 Example of a sample identification label
      6-8 Example of a chain-of-custody tag or label
      6-9 Example of a chain-of-custody record form
      6-10 Recommended measurements of body length and size for fish, shellfish, and turtles
      7-1 Preparation of fish fillet composite homogenate samples
      7-2 Sample processing record for fish contaminant monitoring program -- fish fillet composites
      7-3 Illustration of basic fish filleting procedure
      7-4 Preparation of individual turtle homogenate samples
      7-5 Sample processing record for a contaminant monitoring progra m -- individual turtle samples
      7-6 Illustration of basic turtle resection procedure
      7-7 Preparation of shellfish edible tissue composite homogenate samples
      7-8 Sample processing record for shellfish contaminant monitoring program - edible tissue composites
      7-9 Example of a fish and shellfish monitoring program sample aliquot record
      7-10 Example of a fish and shellfish monitoring program sample transfer record
      8-1 Recommended contents of analytical standard operating procedures (SOPs)
      9-1 Recommended data reporting requirements for screening and intensive studies
      9-2 Key information fields for the National Fish Tissue Residue Data Repository

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      ACKNOWLEDGMENTS

      This report was prepared by the U.S. Environmental Protection Agency, Office of Water, Fish and Wildlife Contamination Program. The EPA Project Manager for this document was Jeffrey Bigler who provided overall project coordination as well as technical direction. EPA was supported in the development of this document by the Research Triangle Institute (RTI) (EPA Contract Number 68-C7-0056). Pat Cunningham of RTI was the contractor's Project Manager. Preparation of the First Edition of this guidance in 1993 was facilitated by the substantial efforts of the numerous Workgroup members and reviewers listed in Appendix A.

      ACRONYMS

      AFS American Fisheries Society
      ANOVA Analysis of Variance
      ATSDR Agency for Toxic Substances and Disease Registry
      BCF bioconcentration factor
      BW body weight
      CDD chlorodibenzo-p-dioxin
      CDF chlorodibenzofuran
      CERCLA Comprehensive Environmental Response, Compensation, and Liability Act
      CLP Contract Laboratory Program
      COC chain-of-custody
      CR consumption rate
      CRM certified reference material
      CRADAs Cooperative Research and Development Agreements
      CSF cancer slope factor
      CSFII Continuing Survey of Food Intake by Indivdiuals
      CSOs combined sewer overflows
      CV coefficient of variation
      CVAAS cold vapor atomic absorption spectrometry
      DFTPP decafluorotriphenylphosphine
      DOT U.S. Department of Transportation
      EMAP Environmental Monitoring and Assessment Program
      EMAP-NC Environmental Monitoring and Assessment Progra m-- Near Coastal
      EPA U.S. Environmental Protection Agency
      EMMI Environmental Monitoring Methods Index System
      FDA U.S. Food and Drug Administration
      FWS U.S. Fish and Wildlife Service
      ¡-BHC benzene hexachloride
      ¡-HCH hexachlorocyclohexane
      GC/ECD gas chromatography/electron capture detection
      GC/FID gas chromatography/flame ionization detection
      GC/FPD gas chromatography/flame photometric detection
      GC/NPD gas chromatography/nitrogen-phosphorus detection
      GC/MS gas chromatography/mass spectrometry
      GFAA graphic furnace atomic absorption spectrometry
      GLIFWC Great Lakes Indian Fish and Wildlife Commission
      GPS Global Positioning System
      HAA hydride generation atomic absorption spectrometry
      HEAST Health Effects Assessment Summary Tables
      HPLC/MS high-performance liquid chromatography/mass spectrometry
      HRGC/LRMS high-resolution gas chromography/low-resolution mass spectrometry
      HRGC/HRMS high-resolution gas chromatography/high-resolution mass spectrometry
      ICP inductively coupled plasma atomic emission spectrometry
      IDL instrument detection limit
      IRIS Integrated Risk Information System
      IUPAC Information Union of Pure and Applied Chemistry
      LAN local area network
      LLD lower limits of detection
      LOAEL lowest observed adverse effects level
      LOD limit of detection
      LOQ limit of quantitation
      MDL method detection limit
      MQL method quantitation limit
      NAS National Academy of Sciences
      NERRS National Estuarine Research Research System
      NCBP National Contaminant Biomonitoring Program
      NCR no-carbon-required
      ND not detected
      NEP National Estuary Program
      NFTDR National Fish Tissue Data Repository
      NIST National Institute of Standards and Technology
      NLFWA National Listing of Residue Fish and Wildlife Advisors
      NOAA National Oceanic and Atmospheric Administration
      NOAEL no observed adverse effects level
      NRCC National Research Council of Canada
      NS&T National States and Trends Program
      NSCRFN National Study of Chemical Residues in Fish
      NTIS National Technical Information Service
      OAPCA Organotin Antifouling Paint Control Act
      OAQPS Office of Air Quality Planning and Standards
      OCDD octachlorodibenzo-p-dioxin
      OCDF octachlorodibenzofuran
      ODES Ocean Discharge Evaluation System
      ODW Office of Drinking Water
      OHEA Office of Health and Environmental Assessment
      OPP Office of Pesticide Programs
      ORSANCO Ohio River Valley Water Sanitation Commission
      PAB Population Adjusted Dose
      PAHs polycyclic aromatic hydrocarbons
      PBBs polybrominated biphenyls
      PCBs polychlorinated biphenyls
      PCDDs polychlorinated dibenzo-p-dioxins
      PCDFs polychlorinated dibenzofurans
      PEC potency equivalency concentration
      PNAs polynuclear aromatic hydrocarbons
      PQL practical quantitation limit
      PTFE polytetrafluoroethylene
      QA quality assurance
      QC quality control
      RCRA Resource Conservation and Recovery Act
      RDL reliable detection limit
      RFs response factors
      RfD reference dose
      RL risk level
      RPs relative potencies
      RPD relative percent difference
      RRFs relative response factors
      RSD relative standard deviation
      SOPs standard operating procedures
      SRMs standard reference materials
      SVs screening values
      2,4,5-T 2,4,5-trichlorophenoxyacetic acid
      2,3,7,8-TCDD 2,3,7,8-tetrachlorodibenzo-p-dioxin
      2,3,7,8-TCDF 2,3,7,8-tetrachlorodibenzofuran
      2,4,5-T 2,4,5-trichlorophenoxyacetic acid
      2,4,5-TCP 2,4,5-trichlorophenol
      TEFs toxicity equivalency factor
      TEQs toxicity equivalency concentrations
      TVA Tennessee Valley Authority
      UF uncertainty factor
      UM modifying factor
      USDA U.S. Department of Agriculture
      USGS U.S. Geological Survey
      USFWS U.S. Fish and Wildlife Service
      WHO World Health Organization

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For more information on EPA's Fish Advisory Program, contact: Jeffrey Bigler at US EPA, 1200 Pennsylvania Ave., NW (4305T), Washington, DC 20460; e-mail: bigler.jeff@epa.gov

 
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