|
|
|
Display category headings
Research Project:
MOLECULAR MECHANISMS OF LIGHT PERCEPTION,SIGNALING& CONTROL OF GENE EXPRESSION BY THE PHYTOCHROMES
Location:
Plant Gene Expression Center (Albany, Ca)
Project Number: 5335-21000-017-00
Project Type:
Appropriated
Start Date: Oct 01, 2000
End Date: Sep 30, 2005
Objective:
To define molecular mechanisms by which the phytochrome (phy) family of photoreceptors perceive informational light siganls from the environment and transduce them to photoresponsive nuclear genes, thereby controlling plant growth and development. Immediate objectives are: (1) Identify signal transduction components in the phyD and phyE pathways and (2) Map the transcriptional network involved in the regulation of photoresponsive by phyD and phyE.
Approach:
The experimental approaches will include: forward genetic screening for mutants defective in phyD or phyE signaling, followed by cloning of the loci; screening for proteins that interact directly with the phyD or phyE photoreceptors using the yeast two-hybrid system, coupled with subsequent reverse-genetic testing for functional involvement of such interactors in the relevant signaling pathways; and microarray analyses of global gene expression profiles in phyD and phyE mutants, directed toward identifying primary transcriptional targets of the two signaling pathways. The identification of components in the signaling circuitry of these photoreceptor systems will provide potential molecular targets and tools for engineering agronomically superior crops. REPLACES 5335-21000-010-00D (3/01). BSL-1; 7/1/04
|
|
Publications
| Related National Programs |
|
|
|
|
Email this page
