Project Number: 5325-32000-005-00
Project Type: Appropriated

Start Date: Jun 13, 2002
End Date: May 31, 2006

Objective:
1. To identify factors influencing survival and re-growth of human pathogenic bacteria in a dairy system, including transfer of pathogens from manure and manure by-products to crops. 2. To identify bacterial populations in the manure environment. 3. To apply this information for designing pathogen control strategies that will be both effective and dairy-friendly.

Approach:
Our overall approach will be to examine pathogenic and non-pathogenic bacteria presence, and their re-growth and transfer to agricultural crops during commercial dairy operations, test these hypotheses in laboratory/greenhouse studies using pathogens with bioluminescent and or antibiotic resistant markers and then evaluate laboratory based pathogen control technologies in field trials at commercial dairies. Rapid, sensitive pathogen detection methodologies that are currently available will be adopted for evaluating the life cycle of pathogen transmission on a typical California dairy. Methods for environmental detection of MAP and for pathogens in bio-aerosols and fog will be developed during these investigations. Electroflotation: Design and evaluate bench-scale prototypes and apply to dairy lagoon fluids and process samples to define contaminant removal in relation to substrate composition selection of electrode, electrolytes (chloride, nitrate, nitrite, sulfate, etc.). FORMERLY:5325-32000-002-00D; 5325-42000-023-00D. 5325-42000-028-00D combined into this project(4/04).

Project Team

Ravva, Subbarao McGarvey, Jeffery Hernlem, Bradley

Project Annual Reports

FY 2003   FY 2002

Related National Programs

Food Safety, (animal and plant products) (108)