Project Number: 5335-22000-006-00
Project Type: Appropriated

Start Date: Dec 23, 2002
End Date: Dec 22, 2007

Objective:
The overall objectives are to characterize the N disease resistance gene and understand the molecular nature of the N gene to elucidate how R gene products recognize pathogens, transduce signals, and how plant defense responses block pathogen growth. The specific objectives are to determine the functional relationship of the N encoded gene, determine the structural and functional relationship of the N encoded protein domains, and isolate NR resistance gene from N repanda.

Approach:
Characterization of N gene expression and structure function relationships will include construction of Ti-plasmid vectors bearing in-vitro constructed versions of the N gene, generation of transgenic plants bearing these N alleles using Agrobacterium tumefaciens-mediated transformation procedures, and challenge of transgenic plants and their progeny with TMV to assess the TMV resistance activity encoded in each N gene construct. The NR gene of N. repanda will be isolated using homology based screening of a lambda library and PRC approaches to amplify NR gene sequences. Once isolated the NR gene will be characterized. BSL-1; 7/1/04 REPLACES: 5335-22000-005-00D (12/02).

Project Team

Baker, Barbara

Project Annual Reports

FY 2003