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Research Project:
CHARACTERIZATION OF EXPRESSION PATTERNS OF STRESS TOLERANCE GENES IN WHEAT
Location:
Wheat Genetics, Quality Physiology and Disease Research
Project Number: 5348-21430-003-00
Project Type:
Appropriated
Start Date: Jun 18, 2003
End Date: Jun 17, 2008
Objective:
Determine biological and molecular processes that improve stress tolerance in wheat, primarily cold stress. The specific objectives are: Characterize expression level changes of genes from known metabolic pathways in wheat plants exposed to stress factors, primarily cold stress. Use a wheat diallel across to characterize the genetic control of the accumulation of metabolic compounds shown to accumulate to higher levels in response to cold exposure, and genetic control of physiological changes associated with response to temperature, such as rates of acclimation and deacclimation. Develop wheat cell lines or
whole plants with artificially altered levels of metabolites shown to be
correlated with stress tolerance in other plant systems, and test for induction of stress tolerance.
Approach:
Use microarrays to analyze the expression levels of genes in the known metabolic pathways of wheat plants as they react to cold stress. Use a wheat diallel cross to characterize the genetic control of the accumulation of metabolic compounds shown to accumulate to higher levels in response to cold exposure, and genetic control of physiological changes associated with response to temperature. Develop wheat cell lines with artificially altered levels of metabolites shown to be correlated with stress tolerance in other plant systems, and test for
induction of stress tolerance. BL-I; 12/16/2002. Replaces 5348-21430-002-00D (6/03).
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