Project Number: 5348-22000-012-00
Project Type: Appropriated

Start Date: Jul 01, 2003
End Date: Apr 30, 2008

Objective:
Identify and characterize the genetic determinants and molecular mechanisms active in suppression of root pathogens of wheat and barley by antagonistic microorganisms; genetically improve the biocontrol activity of antagonistic bacteria; improve understanding of ecology & genetics of antagonistic-root-pathogen interaction; genetically and physiologically characterize populations of root pathogens; identify new sources of genetic resistance to root diseases & develop commercially-usable biocontrol technology.

Approach:
Biological control of root pathogens Gaeumannomyces, Pythium, Rhizoctonia and Fusarium by naturally occurring and genetically engineered microorganisms will be developed and quantified in different soils. The genetic determinants and molecular mechanisms responsible for root colonization and pathogen suppression will be characterized with emphasis on the genetics and regulation of phenazine and phloroglucinol biosynthesis. Structural loci for these anti-fungal metabolites will be used to engineer improved biocontrol agents. The genetic and physiological diversity of populations of the root pathogens, and influence of cropping system on pathogen populations and diversity will be determined. New sources & mechanisms of resistance will be identified. Practical root diseases control will be accomplished by both maximizing the activity of natural biocontrol agents. BL-1; 03/24/03. Replacing 5348-22000-008-00D (6/03).