Project Number: 5354-21220-015-00
Project Type: Appropriated

Start Date: Jul 16, 2003
End Date: Jul 15, 2008

Objective:
Genetic studies and breeding of new germplasm resistant to Meloidogyne spp., trichodorid, aphid and fungal vectored viruses, and late blight. Genetic studies and development of high antioxidant potatoes. Genetic studies and breeding of resistance to viruses of potatoes. Development of transgenic resistance to potato viruses in established potato cultivars utilizing genetic constructs that are in the public domain. Characterize the molecular basis of potato disease resistance to provide gene discovery, targets for molecular manipulation, molecular markers for selection and optimal use of induced defenses. Elucidate the etiological and epidemiological factors that control survival and dissemination of potato viruses and design and implement new and improved control strategies based on knowledge of these factors.

Approach:
Identification of sources of resistance to pathogens and pests accompanied by introgression and breeding to incorporate them into advanced parents suitable for breeding. Analysis and identification of high antioxidant potatoes accompanied by genetic studies and breeding to provide commercially viable materials for industry. Mapping of genomic position of root-knot nematode resistance by use of polymorphism rich markers systems. Production of virus resistant transgenic versions of established varieties by Agrobacterium-mediated transformation. Elucidation of molecular and metabolic signatures of plant defense strategies through gene expression analysis. Discovery of etiological and epidemiological basis of virus and phytoplasma infection of potato through elucidation of genomic structure of variants of pathogens, development of diagnostic techniques and examination of virus reservoirs, weed hosts, and vector species. Replacing 5354-21220-009-00D 6/2003.