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Analytical MethodsWhole Effluent Toxicity (WET) MethodsEPA promulgated a final regulation (60 FR 53529, October 16, 1995) concerning the use of whole effluent toxicity (WET) test methods toprotect aquatic life in National Pollutant Discharge Elimination System (NPDES) data gathering and compliance monitoring under the Clean Water Act. WET is defined as the aggregate toxic effect of an effluent or receiving (ambient) water measured directly with a toxicity test. The Agency-approved WET methods are specified at 40 CFR 136.3, TableIA. These WET methods employ standardized, freshwater, marine, and estuarine vertebrates, invertebrates, and plants to directly measure the acute or short-term chronic adverse effects of effluents and receiving waters monitored under NPDES permits. On November 19, 2002, EPA revised these methods and made available updated method manual editions. Updated Method Manuals
Final Rule
Proposed RuleWhole Effluent Toxicity Test Methods; Extension of Comment Period (November 23, 2001) Fact Sheet -- Extension of Comment Period (November 2001) Fact Sheet - Proposed Rule (October 2001) Proposed Changes to Whole
Effluent Toxicity Method Manuals (PDF, 365KB) Supporting InformationFinal Report: Interlaboratory Variability Study of EPA Short-term Chronic
and Acute Whole Effluent Toxicity Test Methods, Method Guidance and Recommendations for Whole Effluent Toxicity (WET) Testing (40 CFR Part 136) (July 28, 2000) Whole Effluent Toxicity (WET) Test Methods to Protect Aquatic Life (Federal Register Notice) (October 16, 1995) Whole Effluent Toxicity Methods Variability Studies and Guidance Documents Adobe Acrobat Reader is required to view PDF documents. The most recent version of the Adobe Acrobat Reader is available as a free download. An Adobe Acrobat plug-in for assisted technologies is also available.
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Wastewater Management | Drinking Water | Wetlands, Oceans, and Watersheds |
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