USEPA Manual of Methods Manual of Methods for Virology

The electronic text form of the USEPA MANUAL OF METHODS FOR VIROLOGY - EPA publication EPA/600/4-84/013 - is composed of 16 chapters. These chapters are are presented here in Acrobat PDF format.

• Chapter 1. Introduction
• Chapter 2. Cleansing Laboratory Ware and Equipment
• Chapter 3. Sterilization and Disinfection
• Chapter 4. Quality Assurance
• Chapter 5. Virus Adsorption-Elution (Viradel) Disc Filter Procedures for Recovering Viruses from Sewages, Effluents, and Waters
• Chapter 6. Virus Adsorption-Elution (Viradel) Cartridge Filter Procedures for Recovering Viruses from Sewage, Effluents, and Waters
• Chapter 7. Method for Recovering Viruses from Sludges (and Other Solids)
• Chapter 8. Method for Reduction of Cytotoxicity of Sample Concentrates
• Chapter 9. Cell Culture Preparation and Maintenance
• Chapter 10. Cell Culture Procedures for Assaying Plaque-Forming Viruses
• Chapter 11. Virus Plaque Confirmation Procedures
• Chapter 12. Identification of Enteroviruses
• Chapter 13. Data Analysis and Experimental Design (not yet available in PDF format)
• Chapter 14. Concentration and Processing of Waterborne Viruses by Positive Charge 1MDS Cartridge Filters and Organic Flocculation
• Chapter 15. Total Culturable Virus Quantal Assay
• Chapter 16. Procedures for Detecting Coliphages

Note: Adobe Acrobat Reader is required to view PDF documents. The most recent version of the Adobe Acrobat Reader is available as a free download. An Adobe Acrobat plug-in for assisted technologies is also available.

This Manual is also available in hardcopy form. The hardcopy form of the Manual can be obtained by contacting:

Branch Chief, Biohazard Assessment Research Branch
Microbiological and Chemical Exposure Assessment Research Division
National Exposure Research Laboratory
U.S. Environmental Protection Agency
26 West M.L. King Dr.
Cincinnati OH 45268 USA

e-mail: ci_nerl_mceard@epa.gov

Original Date of Publication: February 1984
Subsequent Chapter Revision/Addition Dates: 1986, 1987, 1988, 1989, 1991, 1993 , 2001

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