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EPA 540/MR-93/527
Augmented In Situ Subsurface Bioremediation Process™
BIO-REM, Inc.
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Augmented In Situ Subsurface Bioremediation Process™
BIO-REM, Inc.(17K)
ABSTRACT
Augmented In Situ Subsurface Bioremediation Process™
BIO-REM, Inc.
Demonstration Bulletin
EPA 540/MR-93/527
November 1993
The Augmented In Situ Subsurface Bioremediation Process™ developed by BIO-REM,
Inc., uses microaerophilic bacteria and micronutrients (H-10) and surface tension
depressants/penetrants for the treatment of hydrocarbon contaminated soils and
groundwater. The bacteria utilize hydrocarbons as electron acceptors and can
propagate under very low oxygen tensions thus eliminating the need for additional
oxygen sources as well as nutrient treatment. The contaminants are hardy and
can thrive over a wide temperature range. Application of the technology is adaptable
to site specific situations. The Bio-Rem process was evaluated on JP-4 jet fuel
contaminated soils at a former Liquid Fuels Storage Area on Williams Air Force
Base (AFB), Phoenix, Arizona, between March 1992 and June 1993. Testing involved
the preparation and delivery of 100,000 gallons of H-10 slurry using groundwater
from the site to the subsurface soil via a network of over 315 open soil borings.
The treatment area was monitored via periodic soil sampling. Samples were analyzed
for BTEX, TRPH, and TPH utilizing EPA SW846 methodologies. The developers claimed
that organic contaminants in soils between the depths of four to twenty-five
feet would be reduced to less than the project specific cleanup levels.
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Posted February 26, 1999
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