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EnvoroGard™ PCB Test Kit
Millipore, Inc.
Innovative Technology Evaluation Report
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Envirogard PCB Immunoassay Test Kit, Millipore Corporation (548K)
U.S. EPA Superfund Innovative Technology Evaluation (SITE) Program
EnviroGardTM PCB Immunoassay Test Kit
Millipore Corporation
ABSTRACT
The EnvirogardTM polychlorinated biphenyl (PCB) immunoassay test
kit rapidly analyzes for PCB concentrations in soils. Soils samples are extracted
using methanol; extracts and calibration solutions are added to test tubes coated
with antibodies that bind PCB molecules. The soil extracts are washed away after
incubation, and the PCB conjugate is added to the tubes. Unoccupied antibody
binding sites bind the PCB conjugate. Excess PCB conjugate is washed away. An
enzyme substrate and a coloring agent are then added. The color intensity, which
is inversely proportional to PCB concentration in the sample, is compared against
three calibrators of 5, 10, and 50 ppm. Reported detection limits are 0.1 ppb
in water and 0.1 ppm in soil. The test kit was used to screen and quantify PCB
contamination in soils at a solvent extraction system demonstration in Washburn,
Maine. The kit was also demonstrated at a DOE site in Kansas City, MO. Highly
contaminated soils were easily identified, and quantitative tests provided correlation
to contaminant levels obtained by off-site laboratory analyses using EPA Method
8080.
Back to SITE Home Page
Posted June 22, 1999
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