Project Number: 210-22310-002-21
Project Type: Specific C/A

Start Date: May 18, 2001
End Date: Apr 30, 2006

Objective:
To study at a molecular level mechanisms of Bacillus thuringiensis (Bt) in Lepidoptera.

Approach:
Bt resistant insects (Spodoptera frugiperda and Heliothis virescens, and other Lepidoptera as needed) will be selected by exposure of sequential generations of larvae to purified toxin. Mid-gut from insects will be used to isolate total RNA and RT-PCR will be utilized to survey for potential molecular markers of resistance. Comparative studies will be conducted using non-resistant and resistant insects with focus on the production of anti-toxin molecules.

Project Team

Greene, Richard - Rich Cristina Padilla - Principal Investiagor 301-504-4545

Project Annual Reports

FY 2003   FY 2002

Related National Programs

Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)