Project Number: 5306-21000-015-06
Project Type: Specific C/A

Start Date: Mar 01, 2004
End Date: Feb 28, 2009

Objective:
This specific cooperative agreement has two important objectives: 1. To fingerprint and assess genetic diversity and relationships within and among elite germplasm, advanced selections, and officially released cultivars of grape, stonefruits, and nut crops maintained as virus-tested foundation stocks at the Foundation Plant Services (FPS), University of California, Davis. 2. To validate genetic fingerprints of grape germplasm maintained at the USDA National Clonal Germplasm Repository (NCGR), Davis by comparing them with the standard Vitis microsatellite fingerprint database maintained at the FPS.

Approach:
Objective 1: Microsatellite and AFLP markers will be used to fingerprint foundation stocks of fruit and nut crops maintained at the FPS facility. Assays including fingerprinting activities will be performed at the USDA National Clonal Germplasm Repository's laboratory in collaboration with the repository's geneticist. Genescan and genotyper analyses will be performed on the raw microsatellite and AFLP data assembled by the data collection software used with the ABI Prism 310 and 3100 genetic analyzers. The data thus generated will be added to the GRIN database and also be used along with data from the germplasm maintained at the repository to analyze for the overall genetic diversity and differentiation within and among different gene pools. Objective 2: The microsatellite fingerprint data generated on the Vitis collection maintained at the NCGR will be compared with the standard fingerprint database of Vitis maintained at the FPS. A standard set of grape cultivars will be included in all genetic analyzer runs to minimize variation among runs and analyses. Genotyper results are verified for the accuracy of allele calling or binning of each allele in the samples to establish the identity of each allele and genotype. The resultant data is compared with the standard grape fingerprint database maintained at the FPS. Data across genotypes and runs are merged into a spreadsheet format ready for analyses.

Project Team

Aradhya, Mallikarjuna Deborah Golino - Director, Foundation Plant Services 530-752-9392

Related National Programs

Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)