Project Number: 6615-22000-016-16
Project Type: Specific C/A

Start Date: Sep 01, 2001
End Date: Aug 31, 2006

Objective:
The objective of this cooperative research project is to develop and improve insect gene-transfer methodologies to create transgenic strains for the improvement of beneficial species and the biological control of pest species. The particular method of interest is the delivery of vector DNA to preblastoderm embryos which may be achieved using sperm coated with DNA as a carrier in artificially inseminated females.

Approach:
A major bottleneck for the genetic transformation of many insect species is the ability to deliver vector DNA to preblastoderm embryos with a high level of viability and fertility in resultant adults. New methods for efficient and innocuous DNA delivery will be developed by the Cooperator by testing DNA delivery by artificial insemination with DNA-coated sperm in the honeybee and assessing transient expression of fluorescent protein markers. Germline transformation of the honeybee will then be tested using vector and helper DNA provided by the ADODR. The Cooperator and ADODR will then collaborate in developing artificial insemination techniques for tephritid fruit fly species, with transformation tested by using DNA-coated sperm. BSL-1, April 3, 2004.

Project Team

Sivinski, John Handler, Alfred - Al H. Hall - Associate Professor 352-374-5793

Project Annual Reports

FY 2003   FY 2002

Related National Programs

Crop Protection & Quarantine (304)