|
|
|
Display category headings
Research Project:
Diaprepes Research Block Grant
Location:
Subtropical Insects Research
Project Number: 6618-22000-027-02
Project Type:
Trust
Start Date: Aug 01, 2002
End Date: Jan 31, 2004
Objective:
Determine effect and feasibility of kaolin-based particle sprays for control of the Diaprepes root weevil. Field and laboratory trials will test kaolin sprays for deterrence of feeding and oviposition under simulated and real weathering conditions. Effect of kaolin on nontarget pests and beneficials will be documented. Describe the biological effects of an Iridovirus infection on the development and reproduction of the Diaprepes root weevil. Laboratory trials will describe parameters of infection and pathology and determine conditions for virus propagation within the insect host. Feasibility of virus delivery by infected males to females will be studied through laboratory assays.
Approach:
Laboratory trials will test reaction of adult Diaprepes to kaolin-treated foliage. Leaf consumption and oviposition will be measured in choice and no-choice assays. A precision spray booth will be used to deliver known quantities of kaolin to leaf surfaces to generate a dose response function. Field trials will document long term effects of kaolin on tree growth, productivity, and Diaprepes damage. Periodic counts in the field and laboratory assays, where appropriate, will examine the effect of kaolin on nontarget species. Larvae and adult Diaprepes root weevil will be infected with iridovirus through micro-injection and oral feeding. Inodculated insects will be reared at three different temperatures. Time course studies will determine optimal rearing conditions for virus propagation. Infected insects will be monitored and compared to healthy insects to determine the effects of virus infection on larval survival, adult reproduction, egg survival, and modes of transmission, vertical and horizontal.
|
|
| Related National Programs |
|
|
|
|
Email this page
