Project Number: 6615-22000-016-19
Project Type: Trust

Start Date: Nov 01, 2001
End Date: Oct 30, 2006

Objective:
To create strains of transgenic tephritid fruit flies containing the fluorescent protein markers, GFP and DsRed, that can be used for unambiguous detection and quality control assessment of flies released for biocontrol.

Approach:
GFP and DsRed marker genes regulated by a polyubiquitin promoter will be integrated into wild Caribbean fruit fly (Anastrepha suspensa) and the Mediterannean fruit fly (Ceratitis capitata) TSL genetic-sexing strain D53 by piggyBac transformation methods. Integrations will be mapped and quality control and stability tests will be performed in transformant strains. Medfly strains having integrations that map to chromosome 5 will be tested further for eventual use in mass release programs. BSL-1; June 18,2001

Project Team

Handler, Alfred - Al

Project Annual Reports

FY 2003

Related National Programs

Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)   Crop Protection & Quarantine (304)