Project Number: 6618-22000-028-08
Project Type: Trust

Start Date: Aug 01, 2003
End Date: Jul 31, 2008

Objective:
Determine effect and feasibility of kaolin-based particle sprays for control of the Diaprepes root weevil. Field and laboratory trials will test kaolin sprays for deterrence of feeding and oviposition under simulated and real weathering conditions. Effect of kaolin on nontarget pests and beneficials will be documented. Describe the biological effects of microbial pathogens on the development and reproduction of the Diaprepes root weevil to test the effect of genes that encode inhibitors of digestive enzymes against Diaprepes larvae. Field trials will compare advanced hybrid citrus rootstocks at contrasting locations and levels of of infestation by Diaprepes.

Approach:
Field trials will document long term effects of kaolin on tree growth, productivity, and Diaprepes damage. Periodic counts in the field and laboratory assays, where appropriate, will examine the effect of kaolin on nontarget species. Field trials will be established at four locations in Florida under a split-plot design to quantify edaphic adaptation and tolerance to Diaprepes feeding. Isolates of Bacillus thuringiensis will be tested for pathogenicity and toxicity to Diaprepes larvae. A model plant (alfalfa) will be transformed for expression of TMOF (an inhibitor of insect digestive enzymes) and tested for gene translation and effect of gene product on larvae.

Project Team

Lapointe, Stephen Hunter, Wayne Bowman, Kim Shatters, Robert - Bob Weathersbee, Albert - Allen

Related National Programs

Crop Protection & Quarantine (304)