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Research Project:
CHARACTERIZATION OF THE BIOCHEMICAL BASIS OF HOST SPECIFICITY FOR PENICILLIUM DIGITATUM AND P. ITALICUM ON CITRUS FRUIT
Location:
Subtropical Plant Pathology Research
Project Number: 6618-22000-026-04
Project Type:
Reimbursable
Start Date: Dec 01, 2003
End Date: Nov 30, 2006
Objective:
Identify compounds in citrus peel that stimulate or inhibit germination of fungal pathogens. Determine how levels of these compounds change during fruit development and correlate with resistance. Determine the effect of citrus genotype on levels of the compounds of interest. Develop a mixture of stimulatory compounds and determine how this could be used for decay control.
Approach:
Bioassay directed purification will be used to identify the stiumulatory compounds. Compounds will be isolated by HPLC and identified by NMR. Gene expression studies will be conducted to identify genes in penicillium that are activated by the stimulatory compounds. Susceptibility of fruit of different genetic types at different stages of development will be assessed by challenge inoculation.
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