Project Number: 5442-21220-010-26
Project Type: Specific C/A

Start Date: Jun 01, 2003
End Date: May 31, 2006

Objective:
1) Genetically transform isolates of S. sclerotiorum with the green flourescent protein gene; and 2) detect and quantify the growth of the pathogen in the host tissue.

Approach:
Five isolates of S. sclerotiorum originating from dry bean, soybean, sunflower, canola, and lentil will be used in transformation experiments. A variety of plasmid vectors with the green fluorescent protein gene have been obtained and an attempt will be made to obtain an additional vector for transformation. Plasmid DNA will be produced and a gene gun used for particle bombardments. Southern blotting and hybridization will be accomplished. All transformants will be grown on susceptible crop cultivars to determine if the pathogen has maintained pathogenicity and to determine if the reporter gene markers can be observed on and in the tissue of those hosts.

Project Team

Chandler, Laurence - Larry Berlin Nelson - Plant Pathologist 701-239-1370

Project Annual Reports

FY 2003

Related National Programs

Plant Diseases (303)