Project Number: 3655-22000-017-02
Project Type: Specific C/A

Start Date: Jun 15, 1999
End Date: Jun 14, 2004

Objective:
The objective of this cooperative researach project is to determine the effect of mutations in genes in the gac regulon on field fitness of Pseudomonas syringae pv. syringae.

Approach:
Field experiments will be conducted to determine the role(s) and effects of genes in the gac regulon on interactions of P. syringae pv. syringae with its susceptible host. The experiments will be conducted at the University of Wisconsin-Madison Experiment Station at Arlington, WI. Snap bean plants will serve as the susceptible host. Marked mutant and wild type strains of P. syringae will be inoculated onto bean seeds at the time of planting. Bacterial population sizes and amount of brown spot disease will be determined throughout the growing season. Test strains will include mutations in gac-regulated genes required for brown spot lesion formation (e.g., gacS, salA), syringomycin production (e.g., salA, syrB, syrD) and homoserine lactone production (e.g., ahII). The effects of non-lesion forming mutants such as a salA mutant on minimizing spread and colonization of bean plants by pathogenic P. syringae will also be examined. BSL-1; Recertified 4/3/03 through 4/3/05.

Project Team

Willis, David Susan Hirano - Research Scientist (608)263-2092

Project Annual Reports

FY 2003   FY 2001   FY 2000   FY 1999

Related National Programs

Plant Diseases (303)