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Research Project:
Processes of Underlying Vegetable Nutrient Content, Shelf-Life & Stress Tolerance
Location:
Vegetable Laboratory
Project Number: 1275-21000-155-00
Project Type:
Appropriated
Start Date: Nov 07, 2001
End Date: Aug 31, 2006
Objective:
The goals of this research are to develop:basic information critical for developing vegetable crops for longer shelf life, better nutritional quality, and the ability to withstand environmental extremes; knowledge of mechanisms that regulate calcium accumulation in potato tuber under natural growth conditions. Includes genetic manipulation of anti-oxidant levels in vegetable crops (e.g., tomato) and increasing the ability of the potato plant to sequester calcium in the interior tissue of the tuber.
Approach:
Transgenic tomato fruits homozygous to the introduced S-adenosylmethionine decarboxylase-E8 gene and which produce high levels of polyamines and lycopene were previously developed. We will identify up-regulated and down-regulated genes in these transgenic tomatoes, using DNA microarrays and subtractive cloning technologies, in conjunction with quantitative northern blots. The studies on differential gene expression will be complemented by biochemical analysis to generate information on interactions between polyamines and ethylene. Flux of radiolabeled precursors of ethylene (methionine) and polyamines (arginine and ornithine) will also be studied to test if a relationship exists between polyamine accumulation, chromoplast development and metabolism of a key chloroplast protein D1. Chloroplast-chromoplast differentiation process during fruit ripening will also be studied. Generate research strategies using tools of genomics and molecular biology, to increase in a sustainable manner calcium content of the potato tuber. BL-1, BL1-P; 9/24/01
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