Project Number: 1275-22000-196-03
Project Type: Reimbursable

Start Date: Oct 01, 2003
End Date: Sep 30, 2004

Objective:
1. Identify protein partners that interact with SCN Hsp90 to aid in its function during development. 2. Further define how Hsp90 interferes with hatching, development, or longevity of nematodes. 3. Determine whether disruption of SCN Hsp90 within soybean plants will interfere with the ability of the nematode to establish an infection. 4. Examine gene structure and expression of Hsp90 in selected SCN populations.

Approach:
1. Test the interaction of various SCN proteins with Hsp90 using a yeast two-hybrid system. 2. Test the effect of Hsp90 inhibitors on development, lifespan, and behavior in nematode bioassays. 3. Transform soybean plants to express small RNA molecules that may interefere with Hsp90 function in nematodes, thereby preventing infection of the plant. 4. Use Hsp90 based real-time PCR assays to assess genetic diversity of SCN.

Project Team

Skantar, Andrea

Related National Programs

Plant Diseases (303)