Project Number: 1275-22000-196-04
Project Type: Reimbursable

Start Date: Oct 01, 2004
End Date: Sep 30, 2005

Objective:
1. Conduct studies to further define how Hsp90 interferes with hatching, development, or longevity of nematodes. 2. Determine efficacy of Steptomyces that produce the Hsp90 inhibitor geldanamycin (GA) for disruption of soybean cyst nematode infection on soybean. 3. Determine effect of disrupting SCN Hsp90 within soybean plants on infection on soybean by SCN. 4. Identify and characterize additional genes involved in SCN development and stress responses.

Approach:
Determine effect of GA or heat shock on Hsp90 function in select C. elegans mutants to identify the affected biochemical pathways. 2. Measure the effect of Streptomyces bacterial extracts which contain geldanamycin soybean cyst nematode hatching and motility. 3. Soybean plants that produce inhibitory RNA molecules specific for Hsp90 will be tested for nematode resistance. 4. Use bioinformatics and cDNA library screens to identify candidate genes for further cloning and expression analysis.

Project Team

Skantar, Andrea

Related National Programs

Plant Diseases (303)