Project Number: 1275-21000-164-13
Project Type: Reimbursable

Start Date: Apr 01, 2003
End Date: Mar 31, 2004

Objective:
The first objective is the development of DNA markers called Single Nucleotide Polymorphisms or SNPs to speed soybean variety development using marker assisted selection. The second objective is to map the newly developed SNP markers on the of Univ. of Utah soybean genome map.

Approach:
The development of SNP DNA markers will focus on the discovery of markers from 1) Monsanto BAC-end sequence data and 2) Expressed Sequence Tag (EST) DNA sequence data generated by the USDA, Ames, IA with N.C. Region Soybean Research funding. Polymerase chain reaction primers will be developed using these two sources of soybean DNA sequence data. The SNP markers will be developed via the sequence analysis of a set of six previously identified soybean genotypes which are particularly useful for the discovery of SNPs. Genetic mapping in two of the University of Utah recombinant inbred line mapping populations will be used to position the markers on the soybean genome map along with more than 1000 SSR markers that have been previously mapped. Using this approach as well as targeted marker development we will identify additional SNP markers in close proximity to genes that provide resistance to Phytophthora root rot, foliar insect feeding, and elevated seed protein in order to select new varieties with improved resistance and seed quality characteristics.

Project Team

Cregan, Perry Shoemaker, Randy

Project Annual Reports

FY 2003

Related National Programs

Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)   Plant Biological and Molecular Processes (302)