Project Number: 1275-21000-164-15
Project Type: Reimbursable

Start Date: Feb 01, 2004
End Date: Jan 30, 2006

Objective:
The overall objective of this research is to 'anchor' the physical map of the soybean genome to the genetic map. The specific objectives to accomplish the overall objective are 1) to identify genetic markers that will serve as anchors to the genetic map, 2)to identify bacterial artificial chromosome (BAC) clones in which these markers reside, and 3)to develop and map additional genetic markers as needed to anchor any remaining contigs of BAC clones that are not anchored to the genetic map.

Approach:
The DNA sequence of clones that contain simple sequence repeat (SSR) motifs from which SSR markers were developed and mapped on the soybean genetic map will be used as a source of DNA sequence to develop overgo (OVERlapping oliGOnucleotides) probes. The overgo probes will be hybridized to membranes containing more than 90,000 BAC clones. The clones identified in this manner will serve as anchors to the genetic map. In another project the same 90,000 BAC clones will be fingerprinted and assembled into contigs. Those contigs that are not anchored to the genetic map (floating contigs) will be identified. The DNA sequence of the ends of four to six BAC clones from each floating BAC contig will be determined. Sequence based genetic markers, referral to a single nucleotide polymorphisms (SNPs) will be developed from the sequence information. The SNPs will then be used as genetic markers to map the floating BAC contigs to the genetic map using the single base extension SNP detection system.

Project Team

Cregan, Perry

Related National Programs

Plant, Microbial & Insect Genetic Res., Genomics, & Genetic Improv. I (301)   Plant Biological and Molecular Processes (302)