Project Number: 3625-32000-063-00
Project Type: Appropriated

Start Date: Oct 27, 2001
End Date: Oct 26, 2006

Objective:
Develop and evaluate improved tests for diagnosis of Mycobacterium bovis infection in different animal species; Develop improved methods for differentiation of M. bovis isolates; Characterize M. bovis infection in domestic livestock and wildlife.

Approach:
Sensitivity and specificity of tests for detection of M. bovis infection in live animals will be determined. We will determine if new antigens can be used to improve skin tests and in-vitro diagnostic tests. Improved PCR assays for direct detection of M. bovis in various specimens will be developed by modification of existing tests. Improved methods for isolation of M. bovis from various samples will be developed by changing processing methods and decontaminants. Improved methods for DNA fingerprinting of M. bovis isolates will be developed by adapting published methods. Animals will be exposed to M. bovis by different routes and clinical signs, immune system parameters, and lesion distribution will be monitored. Routes of transmission of M. bovis from infected animals to uninfected animals will be assessed by periodic sampling of oral and nasal secretions, urine, and feces. BSL-3; Approved 10/15/2003 (IBC-#0239).

Project Team

Palmer, Mitchell Waters, Wade Whipple, Diana

Project Annual Reports

FY 2003   FY 2002