Project Number: 500-42-001-26
Project Type: Grant

Start Date: Apr 01, 2001
End Date: Mar 31, 2005

Objective:
Determine the virulence gene and RAPD polymorphism complexity within zoospore-derived isolates obtained from a single parental isolate of P. infestans. Compare virulence gene complexity and RAPD polymorphism of P. infestans parental isolates to the zoospore-derived isolates obtained from them.

Approach:
Hyphal tip isolates of P. infestans collected from throughout the U.S. from 1994 to 2000 and maintained in Rye B agar slants, will be used in this study as parental isolates. Approximately 100 single zoospore isolates will be derived. A differential set of potato plants each carrying a single gene for resistance against P. infestans will be used to determine the virulence structure of these isolates. The potato cultivar Craigs Royal will be used as universal suscept with no known resistance genes. Detached leaf assay tests will be used to assess the virulence of SZIs. Leaflets will be evaluated twice for every disease severity using the disease severity index.

Project Team

Bennett, A. Rick Neil Gudmestad - Professor 301-504-6915

Related National Programs

Plant Diseases (303)   Crop Protection & Quarantine (304)