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Research Project:
Potato Functional Genomics: Application to Analysis of Growth, Development, Metabolism & Stress Responses
Location:
Vegetable and Forage Crops Production Research
Project Number: 5354-21220-002-04
Project Type:
Reimbursable
Start Date: Oct 01, 2002
End Date: Sep 30, 2007
Objective:
Identify markers in a mapping population close to resistance gene to
Columbia Root-knot Nematode derived from Solanum bulbocatanum. Physically
locate position on BAC library. Clone gene(s) involved, and transform into
potato to test for resistance conferring activity.
Approach:
A progeny derived from a cross between resistant and susceptible S.
bulbocastanum parents will be screened for resistance.
AFLP, SCAR and SSR markers will be assayed for linkage to resistance
factors. Markers will be used to identify location on BAC contigs for
chromsome XI, north arm. BACs will be sequence and resistance candidate
genes identified, cloned and inserted into gene shuttle system. Gene
action will be assessed for resistance properties in transgenic plants. Documents Reimbursable with UC Berkeley. Log 21373. Formerly 5354-21220-008-04R, 8/2003.
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