Project Number: 500-42-001-57
Project Type: Grant

Start Date: Apr 06, 2004
End Date: Apr 05, 2005

Objective:
Develop a single assay that can detect and differentiate multiple bacterial potato pathogens and that has the capacity to detect emerging pathogens for which no sequence information is available.

Approach:
Complete proof-of-concept experiment with Erwinia, Pantoea, and Pseudomonas by sequencing the PCR products to confirm that the target sequences have been amplified; determine, using SSCP, if species in these genera can be distinguished; develop a preliminary database based upon the DNA fragment migration profiles; and; determine if this method can detect and differentiate Erwinia in soft rot samples collected and frozen during the potato harvests of 2002 and 2003.

Project Team

Bennett, A. Rick Amy Charkowski - Assistant Professor 301-504-6915

Related National Programs

Plant Diseases (303)