Project Number: 6217-22000-012-03
Project Type: Specific C/A

Start Date: Aug 15, 2001
End Date: Aug 14, 2005

Objective:
Develop species specific PCR primers to amplify 16S rRNA genes in the hyperparasitoid Alloxystra (or other species); use these primers to detect presence of Alloxystra developing within primary aphid parasitoids; validate the effectiveness of Alloxystra primers to accurately determine percent hyperparasitism via laboratory and field experiments; develop PCR primers for differentiation of various cereal aphid hosts (e.g., Schizaphis graminum, Rhopalosiphum padi, and/or Diuraphis noxia).

Approach:
Collect hyperparasitoids from field and identify them to species. Design PCR primers for 16S rRNA genes and sequence the region for comparison with Lysiphlebus testaceipes and aphid hosts. Develop PCR primers specific to Alloxystra 16S rRNA. Evaluate whether the above primers can effectively detect the presence of hyperparasitism of Lysiphlebus testaceipes by Alloxystra in aphid mummies.

Project Team

Shufran, Kevin Yi Chen - Assistant Professor Of Biology 405-624-4141

Project Annual Reports

FY 2003   FY 2002

Related National Programs

Crop Protection & Quarantine (304)   Crop Production (305)